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Heat Shock Proteins

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{{STRUCTURE_3los| PDB=3los | SIZE=400| SCENE= |right|CAPTION=Hsp60 from ''Methanococcus maripaludis '' [[3los]] }}
{{STRUCTURE_3los| PDB=3los | SIZE=400| SCENE= |right|CAPTION=Hsp60 from ''Methanococcus maripaludis '' [[3los]] }}
-
[[Heat Shock Proteins]] (Hsp). The Hsp are proteins which are expressed when cells are exposed to stresses like heat. Hsp60, Hsp70, Hsp90 etc. are named according to their molecular weights. Their function is to provide protection from stress. Hsp contains a nucleotide-binding domain (NBD), a substrate-binding domain (sub) and C-terminal lid domain (lid). In E. coli there are additional Hsp – HslV (19kD), HslJ and HslU (50kD). The heat-inducible Hsp is called '''heat shock cognate protein''' (Hsc).
+
[[Heat Shock Proteins]] (Hsp). The Hsp are proteins which are expressed when cells are exposed to stresses like heat. Hsp60, Hsp70, Hsp90 etc. are named according to their molecular weights. Their function is to provide protection from stress. Hsp contains a nucleotide-binding domain (NBD), a substrate-binding domain (sub) and C-terminal lid domain (lid). In E. coli there are additional Hsp – HslV (19kD), HslJ and HslU (50kD). The heat-inducible Hsp is called '''heat shock cognate protein''' (Hsc). Hsp70 from E. coli is called '''DnaK'''.
See also<br />
See also<br />
* [[Chaperones]]<br />
* [[Chaperones]]<br />
* [[Hsp100]]<br />
* [[Hsp100]]<br />
* [[Hsp40]]
* [[Hsp40]]
-
* [[Molecular Playground/DnaK]] for Hsp70
+
* [[Molecular Playground/DnaK]] for Hsp70 in ''E. coli''
* [[Molecular Playground/sHSP]] for small Hsp
* [[Molecular Playground/sHSP]] for small Hsp
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*Hsp33
*Hsp33
-
**[[1hw7]], [[3m7m]] – EcHsp33 N-terminal <br />
+
**[[1hw7]], [[3m7m]] – EcHsp33 NTD <br />
**[[1i7f]] – EcHsp33 chaperone domain (mutant)<br />
**[[1i7f]] – EcHsp33 chaperone domain (mutant)<br />
-
**[[1xjh]] - EcHsp33 lid - NMR<br />
+
**[[1xjh]] - EcHsp33 CT - NMR<br />
**[[1vq0]] – Hsp33 – ''Thermotoga maritima''<br />
**[[1vq0]] – Hsp33 – ''Thermotoga maritima''<br />
**[[1vzy]] – Hsp33 – ''Bacillus subtilis''
**[[1vzy]] – Hsp33 – ''Bacillus subtilis''
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*Hsp40
*Hsp40
-
**[[1c3g]] – yHsp40 C-terminal peptide binding domain<br />
+
**[[1c3g]] – yHsp40 CT<br />
**[[2qld]] – hHsp40<br />
**[[2qld]] – hHsp40<br />
**[[1hdj]] – hHsp40 J domain - NMR
**[[1hdj]] – hHsp40 J domain - NMR
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**[[1kid]] - EcHsp60 (mutant) – GroEL apical domain<br />
**[[1kid]] - EcHsp60 (mutant) – GroEL apical domain<br />
-
* Hsp70 – 3 domains: N-terminus ATPase hydrolyzes ATP to ADP (NBD); hydrophobic amino acid binding domain (sub): C-terminus lid domain (lid).
+
* Hsp70 – 3 domains: N-terminus ATPase hydrolyzes ATP to ADP (NBD); hydrophobic amino acid binding domain (sub): C-terminus lid domain (CT).
**[[3a8y]] – hHsp70 NBD+BAG domain regulator protein – human<br />
**[[3a8y]] – hHsp70 NBD+BAG domain regulator protein – human<br />
**[[4io8]] - hHsp70 NBD + inhibitor<br />
**[[4io8]] - hHsp70 NBD + inhibitor<br />
-
**[[3lof]] – hHsp70 lid<br />
+
**[[3lof]] – hHsp70 CT<br />
-
**[[4po2]] - hHsp70 lid + peptide<br />
+
**[[4po2]] - hHsp70 CT + peptide<br />
-
**[[2lmg]] – hHsp70 lid - NMR<br />
+
**[[2lmg]] – hHsp70 CT - NMR<br />
**[[1xqs]] – hHsp70 sub + Hsp-binding protein core domain<br />
**[[1xqs]] – hHsp70 sub + Hsp-binding protein core domain<br />
-
**[[2p32]] - CeHsp70 lid<br />
+
**[[2p32]] - CeHsp70 CT<br />
**[[3l6q]] - CpHsp70 NBD – ''Cryptosporidium parvum''<br />
**[[3l6q]] - CpHsp70 NBD – ''Cryptosporidium parvum''<br />
**[[3kvg]] - CpHsp70 NBD+AMPPNP<br />
**[[3kvg]] - CpHsp70 NBD+AMPPNP<br />
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**[[2e8a]] - hHsp70 NBD+AMPPNP<br />
**[[2e8a]] - hHsp70 NBD+AMPPNP<br />
**[[1hx1]] - bHsp70 NBD + molecular chaperone regulator 1 BAG domain<br />
**[[1hx1]] - bHsp70 NBD + molecular chaperone regulator 1 BAG domain<br />
-
**[[2kho]] – EcHsp70 – ''Escherichia coli'' – NMR<br />
 
**[[1q5l]] - EcHsp70 sub +peptide - NMR<br />
**[[1q5l]] - EcHsp70 sub +peptide - NMR<br />
**[[3gl1]] - ScHsp70 NBD – ''Saccharomyces cerevisiae''<br />
**[[3gl1]] - ScHsp70 NBD – ''Saccharomyces cerevisiae''<br />
-
**[[3dpo]] – EcHsp70 sub+12-mer peptide<br />
 
-
**[[3dpp]], [[3dpq]] – EcHsp70 sub+20-mer peptide<br />
 
**[[3dob]], [[3dqg]], [[2op6]] - CeHsp70 sub – ''Caenoharbditis elegans''<br />
**[[3dob]], [[3dqg]], [[2op6]] - CeHsp70 sub – ''Caenoharbditis elegans''<br />
**[[4i2w]] – CeHsp70 + myosin chaperone<br />
**[[4i2w]] – CeHsp70 + myosin chaperone<br />
**[[2d2e]], [[3d2f]] – ScHsp70 NBD+SSE1(Hsp110)<br />
**[[2d2e]], [[3d2f]] – ScHsp70 NBD+SSE1(Hsp110)<br />
**[[2v7y]] – Hsp70 NBD+sub – ''Geobacillus kaustophilus''
**[[2v7y]] – Hsp70 NBD+sub – ''Geobacillus kaustophilus''
 +
 +
*DnaK (Hsp70 from E. coli)
 +
 +
**[[2kho]] – EcDnaK – NMR<br />
 +
**[[1dkg]] - EcDnaK NBD (mutant) + nucleotide exchange factor<br />
 +
**[[4ani]] - EcDnaK NBD +sub + nucleotide exchange factor<br />
 +
**[[4b9q]] - EcDnaK NBD + sub+ ATP<br />
 +
**[[4jn4]], [[4jne]] - EcDnaK NBD + sub (mutant) + ATP<br />
 +
**[[4f01]] - EcDnaK sub<br />
 +
**[[4jnf]] - EcDnaK sub (mutant) + ATP<br />
 +
**[[1bpr]], [[1dg4]], [[2bpr]] - EcDnaK sub – NMR<br />
 +
**[[1q5l]] - EcDnaK sub +peptide – NMR<br />
 +
**[[3dpp]], [[3dpq]], [[3dpo]], [[1dky]], [[3qnj]], [[4e81]], [[4ezn]], [[4ezo]], [[4ezp]], [[4ezq]], [[4ezr]], [[4ezs]], [[4ezt]], [[4ezu]], [[4ezv]], [[4ezw]], [[4ezx]], [[4ezy]], [[4ezz]], [[4f00]], [[4hy9]], [[4hyb]], [[4jwc]], [[4jwd]], [[4jwe]], [[4jwi]], [[4r5i]] – EcDnaK sub+ peptide<br />
 +
**[[4r5j]], [[4r5k]], [[4r5l]] - EcDnaK CT <br />
 +
**[[4r5g]] - EcDnaK CT + inhibitor<br />
 +
**[[4rtf]] DnaK + ATP – ''Mycobacterium tuberculosis''<br />
 +
*Hsp83
*Hsp83
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**[[2qf6]] - hHsp90 NTD + antigen<br />
**[[2qf6]] - hHsp90 NTD + antigen<br />
**[[2xhr]], [[2xht]], [[2xhx]], [[2xjg]], [[2xjj]], [[2xjx]], [[2xk2]], [[2xdx]], [[2xdu]], [[2xds]], [[2xdl]], [[2xdk]], [[2xab]], [[2vcj]], [[2vci]], [[3hyy]], [[3hyz]], [[3hz1]], [[3hz5]], [[3mnr]] - hHsp90 NTD+ antigen<br />
**[[2xhr]], [[2xht]], [[2xhx]], [[2xjg]], [[2xjj]], [[2xjx]], [[2xk2]], [[2xdx]], [[2xdu]], [[2xds]], [[2xdl]], [[2xdk]], [[2xab]], [[2vcj]], [[2vci]], [[3hyy]], [[3hyz]], [[3hz1]], [[3hz5]], [[3mnr]] - hHsp90 NTD+ antigen<br />
-
**[[2brc]], [[2bre]], [[1am1]] - yHsp90 NTD+pyrazol derivative<br />
 
-
**[[1amw]] - yHsp90 NTD+ADP<br />
 
-
**[[1ah6]], [[1ah8]] - yHsp90 NTD<br />
 
**[[1osf]], [[1yer]], [[1yes]], [[1yet]] - hHsp90 NTD+geldamycin <br />
**[[1osf]], [[1yer]], [[1yes]], [[1yet]] - hHsp90 NTD+geldamycin <br />
**[[3ied]] - PfHsp90 NTD+AMPPN – ''Plasmodium falciparum''<br />
**[[3ied]] - PfHsp90 NTD+AMPPN – ''Plasmodium falciparum''<br />
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*Hsp90
*Hsp90
-
**[[1bgq]] – yHsp90+radicicol<br />
 
-
**[[1a4h]], [[2weq]] – yHsp90+ geldamycin<br />
 
**[[1y4s]], [[1y4u]], [[2iop]], [[2ior]] - EcHsp90+ADP<br />
**[[1y4s]], [[1y4u]], [[2iop]], [[2ior]] - EcHsp90+ADP<br />
**[[2ioq]] - EcHsp90 <br />
**[[2ioq]] - EcHsp90 <br />
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* Hsp82 is the yeast equivalent of Hsp90 in mammals
* Hsp82 is the yeast equivalent of Hsp90 in mammals
-
**[[1ah6]], [[1ah8]] – yHsp82 NTD1ah6, 1ah8 – yHsp82 NTD<br />
+
**[[1ah6]], [[1ah8]] – yHsp82 NTD<br />
**[[2xd6]], [[2xx2]], [[2xx4]], [[2xx5]], [[4ce1]], [[4ce2]], [[4ce3]] - yHsp82 NTD+macrolactone<br />
**[[2xd6]], [[2xx2]], [[2xx4]], [[2xx5]], [[4ce1]], [[4ce2]], [[4ce3]] - yHsp82 NTD+macrolactone<br />
 +
**[[1amw]] – yHsp82 NTD+ADP<br />
**[[2wep]] – yHsp82 NTD (mutant)+ADP<br />
**[[2wep]] – yHsp82 NTD (mutant)+ADP<br />
**[[2akp]], [[3c0e]] - yHsp82 NTD (mutant)<br />
**[[2akp]], [[3c0e]] - yHsp82 NTD (mutant)<br />
**[[1weq]], [[3c11]], [[2yga]], [[2wer]], [[2yge]], [[2ygf]] - yHsp82 NTD (mutant)+ antibiotic<br />
**[[1weq]], [[3c11]], [[2yga]], [[2wer]], [[2yge]], [[2ygf]] - yHsp82 NTD (mutant)+ antibiotic<br />
-
**[[2iws]], [[2iwu]], [[2iwx]], [[2cgf]], 1bgq, [[2fxs]], [[4as9]], [[4asa]], [[4asb]], [[4asf]], [[4asg]] - yHsp82 NTD +antibiotic<br />
+
**[[2iws]], [[2iwu]], [[2iwx]], [[2cgf]], 1bgq, [[2fxs]], [[4as9]], [[4asa]], [[4asb]], [[4asf]], [[4asg]], [[2weq]], [[1ah4]], [[1bgq]] - yHsp82 NTD +antibiotic<br />
**[[2vw5]], [[2vwc]] - yHsp82 NTD +7-o-carbamoylpremacbecin<br />
**[[2vw5]], [[2vwc]] - yHsp82 NTD +7-o-carbamoylpremacbecin<br />
**[[2fxs]] - yHsp82 NTD +radamide<br />
**[[2fxs]] - yHsp82 NTD +radamide<br />
**[[1zw9]] - yHsp82 NTD + adenine derivative<br />
**[[1zw9]] - yHsp82 NTD + adenine derivative<br />
**[[1zwh]] - yHsp82 NTD + radester amine<br />
**[[1zwh]] - yHsp82 NTD + radester amine<br />
-
**[[2brc]], [[2bre]] - yHsp82 NTD+pyrazol derivative<br />
+
**[[2brc]], [[2bre]], [[1am1]] - yHsp82 NTD+pyrazol derivative<br />
**[[2cg9]] – yHsp82+co-chaperone SBA1<br />
**[[2cg9]] – yHsp82+co-chaperone SBA1<br />
**[[2cge]] - yHsp82 MD+CT+co-chaperone SBA1<br />
**[[2cge]] - yHsp82 MD+CT+co-chaperone SBA1<br />

Revision as of 09:23, 16 April 2015

Template:STRUCTURE 3los

Heat Shock Proteins (Hsp). The Hsp are proteins which are expressed when cells are exposed to stresses like heat. Hsp60, Hsp70, Hsp90 etc. are named according to their molecular weights. Their function is to provide protection from stress. Hsp contains a nucleotide-binding domain (NBD), a substrate-binding domain (sub) and C-terminal lid domain (lid). In E. coli there are additional Hsp – HslV (19kD), HslJ and HslU (50kD). The heat-inducible Hsp is called heat shock cognate protein (Hsc). Hsp70 from E. coli is called DnaK. See also

3D Structures of Heat Shock Proteins

Updated on 16-April-2015


Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky

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