This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4wm1

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 18:33, 1 February 2017

High pressure protein crystallography of hen egg white lysozyme at 500 MPa

Structural highlights

4wm1 is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4wld, 4wlt, 4wlx, 4wly, 4wm2, 4wm3, 4wm4, 4wm5, 4wm6
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Publication Abstract from PubMed

Crystal structures of hen egg-white lysozyme (HEWL) determined under pressures ranging from ambient pressure to 950 MPa are presented. From 0.1 to 710 MPa, the molecular and internal cavity volumes are monotonically compressed. However, from 710 to 890 MPa the internal cavity volume remains almost constant. Moreover, as the pressure increases to 950 MPa, the tetragonal crystal of HEWL undergoes a phase transition from P43212 to P43. Under high pressure, the crystal structure of the enzyme undergoes several local and global changes accompanied by changes in hydration structure. For example, water molecules penetrate into an internal cavity neighbouring the active site and induce an alternate conformation of one of the catalytic residues, Glu35. These phenomena have not been detected by conventional X-ray crystal structure analysis and might play an important role in the catalytic activity of HEWL.

High-pressure protein crystallography of hen egg-white lysozyme.,Yamada H, Nagae T, Watanabe N Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):742-53. doi:, 10.1107/S1399004715000292. Epub 2015 Mar 26. PMID:25849385^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Yamada H, Nagae T, Watanabe N. High-pressure protein crystallography of hen egg-white lysozyme. Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):742-53. doi:, 10.1107/S1399004715000292. Epub 2015 Mar 26. PMID:25849385 doi:http://dx.doi.org/10.1107/S1399004715000292

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wm1"

@@ Line 1: / Line 1: @@
 ==High pressure protein crystallography of hen egg white lysozyme at 500 MPa==
 <StructureSection load='4wm1' size='340' side='right' caption='[[4wm1]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wld|4wld]], [[4wlt|4wlt]], [[4wlx|4wlx]], [[4wly|4wly]], [[4wm2|4wm2]], [[4wm3|4wm3]], [[4wm4|4wm4]], [[4wm5|4wm5]], [[4wm6|4wm6]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wm1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wm1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wm1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wm1 OCA], [http://pdbe.org/4wm1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wm1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wm1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wm1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4wm1" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4wm1

From Proteopedia

Revision as of 18:33, 1 February 2017

High pressure protein crystallography of hen egg white lysozyme at 500 MPa

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox