2v7g
From Proteopedia
(Difference between revisions)
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|PDB= 2v7g |SIZE=350|CAPTION= <scene name='initialview01'>2v7g</scene>, resolution 2.00Å | |PDB= 2v7g |SIZE=350|CAPTION= <scene name='initialview01'>2v7g</scene>, resolution 2.00Å | ||
|SITE= <scene name='pdbsite=AC1:Nad+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Nad+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Nad+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:Nad+Binding+Site+For+Chain+D'>AC4</scene>, <scene name='pdbsite=AC5:Act+Binding+Site+For+Chain+C'>AC5</scene>, <scene name='pdbsite=AC6:Act+Binding+Site+For+Chain+B'>AC6</scene>, <scene name='pdbsite=AC7:Act+Binding+Site+For+Chain+B'>AC7</scene>, <scene name='pdbsite=AC8:Act+Binding+Site+For+Chain+A'>AC8</scene>, <scene name='pdbsite=AC9:Act+Binding+Site+For+Chain+B'>AC9</scene>, <scene name='pdbsite=BC1:Act+Binding+Site+For+Chain+A'>BC1</scene>, <scene name='pdbsite=BC2:Act+Binding+Site+For+Chain+C'>BC2</scene>, <scene name='pdbsite=BC3:Gol+Binding+Site+For+Chain+B'>BC3</scene>, <scene name='pdbsite=BC4:Gol+Binding+Site+For+Chain+D'>BC4</scene>, <scene name='pdbsite=BC5:Gol+Binding+Site+For+Chain+B'>BC5</scene>, <scene name='pdbsite=BC6:Gol+Binding+Site+For+Chain+D'>BC6</scene>, <scene name='pdbsite=BC7:Gol+Binding+Site+For+Chain+A'>BC7</scene>, <scene name='pdbsite=BC8:Gol+Binding+Site+For+Chain+D'>BC8</scene>, <scene name='pdbsite=BC9:Gol+Binding+Site+For+Chain+B'>BC9</scene>, <scene name='pdbsite=CC1:Gol+Binding+Site+For+Chain+A'>CC1</scene> and <scene name='pdbsite=CC2:Gol+Binding+Site+For+Chain+D'>CC2</scene> | |SITE= <scene name='pdbsite=AC1:Nad+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Nad+Binding+Site+For+Chain+B'>AC2</scene>, <scene name='pdbsite=AC3:Nad+Binding+Site+For+Chain+C'>AC3</scene>, <scene name='pdbsite=AC4:Nad+Binding+Site+For+Chain+D'>AC4</scene>, <scene name='pdbsite=AC5:Act+Binding+Site+For+Chain+C'>AC5</scene>, <scene name='pdbsite=AC6:Act+Binding+Site+For+Chain+B'>AC6</scene>, <scene name='pdbsite=AC7:Act+Binding+Site+For+Chain+B'>AC7</scene>, <scene name='pdbsite=AC8:Act+Binding+Site+For+Chain+A'>AC8</scene>, <scene name='pdbsite=AC9:Act+Binding+Site+For+Chain+B'>AC9</scene>, <scene name='pdbsite=BC1:Act+Binding+Site+For+Chain+A'>BC1</scene>, <scene name='pdbsite=BC2:Act+Binding+Site+For+Chain+C'>BC2</scene>, <scene name='pdbsite=BC3:Gol+Binding+Site+For+Chain+B'>BC3</scene>, <scene name='pdbsite=BC4:Gol+Binding+Site+For+Chain+D'>BC4</scene>, <scene name='pdbsite=BC5:Gol+Binding+Site+For+Chain+B'>BC5</scene>, <scene name='pdbsite=BC6:Gol+Binding+Site+For+Chain+D'>BC6</scene>, <scene name='pdbsite=BC7:Gol+Binding+Site+For+Chain+A'>BC7</scene>, <scene name='pdbsite=BC8:Gol+Binding+Site+For+Chain+D'>BC8</scene>, <scene name='pdbsite=BC9:Gol+Binding+Site+For+Chain+B'>BC9</scene>, <scene name='pdbsite=CC1:Gol+Binding+Site+For+Chain+A'>CC1</scene> and <scene name='pdbsite=CC2:Gol+Binding+Site+For+Chain+D'>CC2</scene> | ||
| - | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Urocanate_hydratase Urocanate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.49 4.2.1.49] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urocanate_hydratase Urocanate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.49 4.2.1.49] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1uwk|1UWK]], [[1uwl|1UWL]], [[1w1u|1W1U]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v7g OCA], [http://www.ebi.ac.uk/pdbsum/2v7g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v7g RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Schulz, G E.]] | [[Category: Schulz, G E.]] | ||
[[Category: Treiber, N.]] | [[Category: Treiber, N.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: NAD]] | ||
[[Category: cytoplasm]] | [[Category: cytoplasm]] | ||
[[Category: histidine degradation]] | [[Category: histidine degradation]] | ||
| Line 36: | Line 36: | ||
[[Category: urocanate hydratase]] | [[Category: urocanate hydratase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:09:13 2008'' |
Revision as of 02:09, 31 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , , , , , , , , , and | ||||||
| Ligands: | , , | ||||||
| Activity: | Urocanate hydratase, with EC number 4.2.1.49 | ||||||
| Related: | 1UWK, 1UWL, 1W1U
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF AN ENGINEERED UROCANASE TETRAMER
Overview
The analysis of natural contact interfaces between protein subunits and between proteins has disclosed some general rules governing their association. We have applied these rules to produce a number of novel assemblies, demonstrating that a given protein can be engineered to form contacts at various points of its surface. Symmetry plays an important role because it defines the multiplicity of a designed contact and therefore the number of required mutations. Some of the proteins needed only a single side-chain alteration in order to associate to a higher-order complex. The mobility of the buried side chains has to be taken into account. Four assemblies have been structurally elucidated. Comparisons between the designed contacts and the results will provide useful guidelines for the development of future architectures.
About this Structure
2V7G is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Reference
Designed protein-protein association., Grueninger D, Treiber N, Ziegler MO, Koetter JW, Schulze MS, Schulz GE, Science. 2008 Jan 11;319(5860):206-9. PMID:18187656
Page seeded by OCA on Mon Mar 31 05:09:13 2008
