2v9p
From Proteopedia
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|PDB= 2v9p |SIZE=350|CAPTION= <scene name='initialview01'>2v9p</scene>, resolution 3.00Å | |PDB= 2v9p |SIZE=350|CAPTION= <scene name='initialview01'>2v9p</scene>, resolution 3.00Å | ||
|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Po4+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Po4+Binding+Site+For+Chain+B'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Chain+B'>AC6</scene>, <scene name='pdbsite=AC7:Po4+Binding+Site+For+Chain+C'>AC7</scene>, <scene name='pdbsite=AC8:Po4+Binding+Site+For+Chain+C'>AC8</scene>, <scene name='pdbsite=AC9:Mg+Binding+Site+For+Chain+C'>AC9</scene>, <scene name='pdbsite=BC1:Po4+Binding+Site+For+Chain+D'>BC1</scene>, <scene name='pdbsite=BC2:Po4+Binding+Site+For+Chain+E'>BC2</scene>, <scene name='pdbsite=BC3:Po4+Binding+Site+For+Chain+F'>BC3</scene>, <scene name='pdbsite=BC4:Po4+Binding+Site+For+Chain+G'>BC4</scene>, <scene name='pdbsite=BC5:Po4+Binding+Site+For+Chain+G'>BC5</scene>, <scene name='pdbsite=BC6:Mg+Binding+Site+For+Chain+G'>BC6</scene>, <scene name='pdbsite=BC7:Po4+Binding+Site+For+Chain+H'>BC7</scene>, <scene name='pdbsite=BC8:Po4+Binding+Site+For+Chain+H'>BC8</scene>, <scene name='pdbsite=BC9:Mg+Binding+Site+For+Chain+H'>BC9</scene>, <scene name='pdbsite=CC1:Po4+Binding+Site+For+Chain+I'>CC1</scene>, <scene name='pdbsite=CC2:Po4+Binding+Site+For+Chain+I'>CC2</scene>, <scene name='pdbsite=CC3:Mg+Binding+Site+For+Chain+I'>CC3</scene>, <scene name='pdbsite=CC4:Po4+Binding+Site+For+Chain+J'>CC4</scene>, <scene name='pdbsite=CC5:Po4+Binding+Site+For+Chain+K'>CC5</scene> and <scene name='pdbsite=CC6:Po4+Binding+Site+For+Chain+L'>CC6</scene> | |SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Mg+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Po4+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Po4+Binding+Site+For+Chain+B'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Chain+B'>AC6</scene>, <scene name='pdbsite=AC7:Po4+Binding+Site+For+Chain+C'>AC7</scene>, <scene name='pdbsite=AC8:Po4+Binding+Site+For+Chain+C'>AC8</scene>, <scene name='pdbsite=AC9:Mg+Binding+Site+For+Chain+C'>AC9</scene>, <scene name='pdbsite=BC1:Po4+Binding+Site+For+Chain+D'>BC1</scene>, <scene name='pdbsite=BC2:Po4+Binding+Site+For+Chain+E'>BC2</scene>, <scene name='pdbsite=BC3:Po4+Binding+Site+For+Chain+F'>BC3</scene>, <scene name='pdbsite=BC4:Po4+Binding+Site+For+Chain+G'>BC4</scene>, <scene name='pdbsite=BC5:Po4+Binding+Site+For+Chain+G'>BC5</scene>, <scene name='pdbsite=BC6:Mg+Binding+Site+For+Chain+G'>BC6</scene>, <scene name='pdbsite=BC7:Po4+Binding+Site+For+Chain+H'>BC7</scene>, <scene name='pdbsite=BC8:Po4+Binding+Site+For+Chain+H'>BC8</scene>, <scene name='pdbsite=BC9:Mg+Binding+Site+For+Chain+H'>BC9</scene>, <scene name='pdbsite=CC1:Po4+Binding+Site+For+Chain+I'>CC1</scene>, <scene name='pdbsite=CC2:Po4+Binding+Site+For+Chain+I'>CC2</scene>, <scene name='pdbsite=CC3:Mg+Binding+Site+For+Chain+I'>CC3</scene>, <scene name='pdbsite=CC4:Po4+Binding+Site+For+Chain+J'>CC4</scene>, <scene name='pdbsite=CC5:Po4+Binding+Site+For+Chain+K'>CC5</scene> and <scene name='pdbsite=CC6:Po4+Binding+Site+For+Chain+L'>CC6</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1f08|1F08]], [[1ksy|1KSY]], [[1ksx|1KSX]], [[2gxa|2GXA]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v9p OCA], [http://www.ebi.ac.uk/pdbsum/2v9p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v9p RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2V9P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2V9P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus_type_1 Bovine papillomavirus type 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V9P OCA]. |
==Reference== | ==Reference== | ||
Papillomavirus E1 helicase assembly maintains an asymmetric state in the absence of DNA and nucleotide cofactors., Sanders CM, Kovalevskiy OV, Sizov D, Lebedev AA, Isupov MN, Antson AA, Nucleic Acids Res. 2007;35(19):6451-7. Epub 2007 Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17881379 17881379] | Papillomavirus E1 helicase assembly maintains an asymmetric state in the absence of DNA and nucleotide cofactors., Sanders CM, Kovalevskiy OV, Sizov D, Lebedev AA, Isupov MN, Antson AA, Nucleic Acids Res. 2007;35(19):6451-7. Epub 2007 Sep 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17881379 17881379] | ||
| - | [[Category: Bovine papillomavirus type | + | [[Category: Bovine papillomavirus type 1]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Antson, A A.]] | [[Category: Antson, A A.]] | ||
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[[Category: Sanders, C M.]] | [[Category: Sanders, C M.]] | ||
[[Category: Sizov, D.]] | [[Category: Sizov, D.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: PO4]] | ||
[[Category: aaa+ molecular motor]] | [[Category: aaa+ molecular motor]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:09:59 2008'' |
Revision as of 02:10, 31 March 2008
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| , resolution 3.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , , , , , , , , , , , , , and | ||||||
| Ligands: | , | ||||||
| Related: | 1F08, 1KSY, 1KSX, 2GXA
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF PAPILLOMAVIRUS E1 HEXAMERIC HELICASE DNA-FREE FORM
Overview
Concerted, stochastic and sequential mechanisms of action have been proposed for different hexameric AAA+ molecular motors. Here we report the crystal structure of the E1 helicase from bovine papillomavirus, where asymmetric assembly is for the first time observed in the absence of nucleotide cofactors and DNA. Surprisingly, the ATP-binding sites adopt specific conformations linked to positional changes in the DNA-binding hairpins, which follow a wave-like trajectory, as observed previously in the E1/DNA/ADP complex. The protein's assembly thus maintains such an asymmetric state in the absence of DNA and nucleotide cofactors, allowing consideration of the E1 helicase action as the propagation of a conformational wave around the protein ring. The data imply that the wave's propagation within the AAA+ domains is not necessarily coupled with a strictly sequential hydrolysis of ATP. Since a single ATP hydrolysis event would affect the whole hexamer, such events may simply serve to rectify the direction of the wave's motion.
About this Structure
2V9P is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.
Reference
Papillomavirus E1 helicase assembly maintains an asymmetric state in the absence of DNA and nucleotide cofactors., Sanders CM, Kovalevskiy OV, Sizov D, Lebedev AA, Isupov MN, Antson AA, Nucleic Acids Res. 2007;35(19):6451-7. Epub 2007 Sep 19. PMID:17881379
Page seeded by OCA on Mon Mar 31 05:09:59 2008
Categories: Bovine papillomavirus type 1 | Single protein | Antson, A A. | Isupov, M N. | Kovalevskiy, O V. | Lebedev, A A. | Sanders, C M. | Sizov, D. | Aaa+ molecular motor | Atp-binding | Atpase | Dna replication | Dna translocation | Dna-binding | Early protein | Helicase | Hydrolase | Nucleotide-binding | Nucleus | Replication
