This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2vb2
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2vb2 |SIZE=350|CAPTION= <scene name='initialview01'>2vb2</scene>, resolution 1.70Å | |PDB= 2vb2 |SIZE=350|CAPTION= <scene name='initialview01'>2vb2</scene>, resolution 1.70Å | ||
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+X'>AC1</scene> and <scene name='pdbsite=AC2:Cu+Binding+Site+For+Chain+X'>AC2</scene> | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+X'>AC1</scene> and <scene name='pdbsite=AC2:Cu+Binding+Site+For+Chain+X'>AC2</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zeq|1ZEQ]], [[2vb3|2VB3]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vb2 OCA], [http://www.ebi.ac.uk/pdbsum/2vb2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2vb2 RCSB]</span> | ||
}} | }} | ||
| Line 31: | Line 34: | ||
[[Category: Stasser, J P.]] | [[Category: Stasser, J P.]] | ||
[[Category: Xue, Y.]] | [[Category: Xue, Y.]] | ||
| - | [[Category: CU]] | ||
| - | [[Category: SO4]] | ||
[[Category: cation pi]] | [[Category: cation pi]] | ||
[[Category: copper]] | [[Category: copper]] | ||
| Line 41: | Line 42: | ||
[[Category: periplasm]] | [[Category: periplasm]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:10:34 2008'' |
Revision as of 02:10, 31 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | , | ||||||
| Related: | 1ZEQ, 2VB3
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF CU(I)CUSF
Overview
Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-pi interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry.
About this Structure
2VB2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Cu(I) recognition via cation-pi and methionine interactions in CusF., Xue Y, Davis AV, Balakrishnan G, Stasser JP, Staehlin BM, Focia P, Spiro TG, Penner-Hahn JE, O'Halloran TV, Nat Chem Biol. 2008 Feb;4(2):107-9. Epub 2007 Dec 23. PMID:18157124
Page seeded by OCA on Mon Mar 31 05:10:34 2008
