2ve6
From Proteopedia
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|PDB= 2ve6 |SIZE=350|CAPTION= <scene name='initialview01'>2ve6</scene>, resolution 2.65Å | |PDB= 2ve6 |SIZE=350|CAPTION= <scene name='initialview01'>2ve6</scene>, resolution 2.65Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=PRQ:(3S)-3-AMINO-3-(2-NITROPHENYL)PROPANOIC+ACID'>PRQ</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00098 IGc], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00129 MHC_I]</span> | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ve6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ve6 OCA], [http://www.ebi.ac.uk/pdbsum/2ve6 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2ve6 RCSB]</span> | ||
}} | }} | ||
'''CRYSTAL STRUCTURE OF A MURINE MHC CLASS I H2-DB MOLECULE IN COMPLEX WITH A PHOTOCLEAVABLE PEPTIDE''' | '''CRYSTAL STRUCTURE OF A MURINE MHC CLASS I H2-DB MOLECULE IN COMPLEX WITH A PHOTOCLEAVABLE PEPTIDE''' | ||
| + | |||
| + | ==Overview== | ||
| + | Class I MHC tetramers allow direct phenotypic identification of CD8(+) T cell populations, but their production remains laborious. A peptide exchange strategy that employs class I MHC products loaded with conditional ligands (caged MHC molecules) provides a fast and straightforward method to obtain diverse arrays of class I MHC tetramers and facilitates CD8(+) T cell epitope discovery. Here, we describe the development of photocleavable analogs of the FAPGNYPAL (SV9) epitope that bind H-2K(b) and H-2D(b) with full retention of their structural and functional integrity. We ranked all possible H-2K(b) octameric and H-2D(b) nonameric epitopes that span the genome of Chlamydia trachomatis and prepared MHC tetramers from approximately 2,000 of the highest scoring peptides by replacement of the SV9 analog with the peptide of choice. The resulting 2,000-member class I MHC tetramer array allowed the discovery of two variants of an epitope derived from polymorphic membrane protein I (PmpI) and an assessment of the kinetics of emergence and the effector function of the corresponding CD8(+) T cells. | ||
==About this Structure== | ==About this Structure== | ||
2VE6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VE6 OCA]. | 2VE6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VE6 OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers., Grotenbreg GM, Roan NR, Guillen E, Meijers R, Wang JH, Bell GW, Starnbach MN, Ploegh HL, Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3831-6. Epub 2008 Feb 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18245382 18245382] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 10:03:11 2008'' |
Revision as of 08:03, 26 March 2008
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| , resolution 2.65Å | |||||||
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| Ligands: | |||||||
| Domains: | IGc, MHC_I | ||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A MURINE MHC CLASS I H2-DB MOLECULE IN COMPLEX WITH A PHOTOCLEAVABLE PEPTIDE
Overview
Class I MHC tetramers allow direct phenotypic identification of CD8(+) T cell populations, but their production remains laborious. A peptide exchange strategy that employs class I MHC products loaded with conditional ligands (caged MHC molecules) provides a fast and straightforward method to obtain diverse arrays of class I MHC tetramers and facilitates CD8(+) T cell epitope discovery. Here, we describe the development of photocleavable analogs of the FAPGNYPAL (SV9) epitope that bind H-2K(b) and H-2D(b) with full retention of their structural and functional integrity. We ranked all possible H-2K(b) octameric and H-2D(b) nonameric epitopes that span the genome of Chlamydia trachomatis and prepared MHC tetramers from approximately 2,000 of the highest scoring peptides by replacement of the SV9 analog with the peptide of choice. The resulting 2,000-member class I MHC tetramer array allowed the discovery of two variants of an epitope derived from polymorphic membrane protein I (PmpI) and an assessment of the kinetics of emergence and the effector function of the corresponding CD8(+) T cells.
About this Structure
2VE6 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers., Grotenbreg GM, Roan NR, Guillen E, Meijers R, Wang JH, Bell GW, Starnbach MN, Ploegh HL, Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3831-6. Epub 2008 Feb 1. PMID:18245382
Page seeded by OCA on Wed Mar 26 10:03:11 2008
Categories: Mus musculus | Protein complex | Bell, G W. | Grotenbreg, G M. | Guillen, E. | Meijers, R. | Ploegh, H L. | Roan, N R. | Starnbach, M N. | Wang, J H. | Auxiliary anchoring residue | Glycoprotein | Immune response | Immune system | Immunoglobulin domain | Membrane | Mhc | Mhc i | Peptide loading | Photocleavable peptide | Polymorphism | Secreted | Sev9 | Transmembrane
