2vh5

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|PDB= 2vh5 |SIZE=350|CAPTION= <scene name='initialview01'>2vh5</scene>, resolution 2.70&Aring;
|PDB= 2vh5 |SIZE=350|CAPTION= <scene name='initialview01'>2vh5</scene>, resolution 2.70&Aring;
|SITE= <scene name='pdbsite=AC1:Gtp+Binding+Site+For+Chain+R'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+R'>AC2</scene> and <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+H'>AC3</scene>
|SITE= <scene name='pdbsite=AC1:Gtp+Binding+Site+For+Chain+R'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+R'>AC2</scene> and <scene name='pdbsite=AC3:Zn+Binding+Site+For+Chain+H'>AC3</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>
+
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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[[Category: signaling protein/immune system]]
[[Category: signaling protein/immune system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:46:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:54:15 2008''

Revision as of 13:54, 23 March 2008

Image:2vh5.jpg


PDB ID 2vh5

Drag the structure with the mouse to rotate
, resolution 2.70Å
Sites: , and
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF HRAS(G12V)- ANTI-RAS FV (DISULFIDE FREE MUTANT) COMPLEX


Overview

Intracellular antibody fragments that interfere with molecular interactions inside cells are valuable in investigation of interactomes and in therapeutics, but their application demands that they function in the reducing cellular milieu. We show here a 2.7-A crystal structure of intracellular antibody folds based on scaffolds developed from intracellular antibody capture technology, and we reveal that there is no structural or functional difference with or without the intra-domain disulfide bond of the variable domain of heavy chain or the variable domain of light chain. The data indicate that, in the reducing in vivo environment, the absence of the intra-domain disulfide bond is not an impediment to correction of antibody folding or to interaction with antigen. Thus, the structural constraints for in-cell function are intrinsic to variable single-domain framework sequences, providing a generic scaffold for isolation of functional intracellular antibody single domains.

About this Structure

2VH5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Functional intracellular antibody fragments do not require invariant intra-domain disulfide bonds., Tanaka T, Rabbitts TH, J Mol Biol. 2008 Feb 22;376(3):749-57. Epub 2007 Dec 4. PMID:18187153

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