2z3j
From Proteopedia
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|PDB= 2z3j |SIZE=350|CAPTION= <scene name='initialview01'>2z3j</scene>, resolution 1.60Å | |PDB= 2z3j |SIZE=350|CAPTION= <scene name='initialview01'>2z3j</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Blasticidin-S_deaminase Blasticidin-S deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.23 3.5.4.23] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2z3g|2Z3G]], [[2z3h|2Z3H]], [[2z3i|2Z3I]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z3j OCA], [http://www.ebi.ac.uk/pdbsum/2z3j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z3j RCSB]</span> | ||
}} | }} | ||
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[[Category: Yamaguchi, I.]] | [[Category: Yamaguchi, I.]] | ||
[[Category: Yamamoto, M.]] | [[Category: Yamamoto, M.]] | ||
| - | [[Category: CAC]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: ZN]] | ||
[[Category: cytidine deaminase family]] | [[Category: cytidine deaminase family]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:18:11 2008'' |
Revision as of 02:18, 31 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Blasticidin-S deaminase, with EC number 3.5.4.23 | ||||||
| Related: | 2Z3G, 2Z3H, 2Z3I
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of blasticidin S deaminase (BSD) R90K mutant
Overview
Blasticidin S deaminase from Aspergillus terreus was crystallized with polyethylene glycol 8000. Two types of crystals were grown under the same crystallization conditions. One type grew as thin plates, while the other had a rhombic shape. The rhombic shaped crystal was suitable for high-resolution crystal structure analysis. Precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56.48 A. The calculated Vm value was acceptable when a tetramer of the enzyme was contained in an asymmetric unit. Preliminary diffraction data were collected to a resolution of 2.0 A with good statistics.
About this Structure
2Z3J is a Single protein structure of sequence from Aspergillus terreus. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:10089374
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