This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2z8f
From Proteopedia
(New page: 200px {{Structure |PDB= 2z8f |SIZE=350|CAPTION= <scene name='initialview01'>2z8f</scene>, resolution 1.65Å |SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+...) |
|||
| Line 4: | Line 4: | ||
|PDB= 2z8f |SIZE=350|CAPTION= <scene name='initialview01'>2z8f</scene>, resolution 1.65Å | |PDB= 2z8f |SIZE=350|CAPTION= <scene name='initialview01'>2z8f</scene>, resolution 1.65Å | ||
|SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+For+Residue+A+4'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+3'>AC2</scene>, <scene name='pdbsite=AC3:Nag+Binding+Site+For+Residue+A+2'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+1'>AC4</scene>, <scene name='pdbsite=AC5:Bgc+Binding+Site+For+Residue+B+4'>AC5</scene>, <scene name='pdbsite=AC6:Gal+Binding+Site+For+Residue+B+3'>AC6</scene>, <scene name='pdbsite=AC7:Nag+Binding+Site+For+Residue+B+2'>AC7</scene>, <scene name='pdbsite=AC8:Gal+Binding+Site+For+Residue+B+1'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Residue+A+11'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Residue+B+12'>BC1</scene>, <scene name='pdbsite=BC2:Zn+Binding+Site+For+Residue+A+13'>BC2</scene>, <scene name='pdbsite=BC3:Zn+Binding+Site+For+Residue+B+14'>BC3</scene>, <scene name='pdbsite=BC4:Zn+Binding+Site+For+Residue+B+15'>BC4</scene>, <scene name='pdbsite=BC5:Na+Binding+Site+For+Residue+B+16'>BC5</scene>, <scene name='pdbsite=BC6:Na+Binding+Site+For+Residue+A+17'>BC6</scene>, <scene name='pdbsite=BC7:Na+Binding+Site+For+Residue+A+18'>BC7</scene>, <scene name='pdbsite=BC8:Mes+Binding+Site+For+Residue+A+21'>BC8</scene> and <scene name='pdbsite=BC9:Mes+Binding+Site+For+Residue+B+22'>BC9</scene> | |SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+For+Residue+A+4'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+3'>AC2</scene>, <scene name='pdbsite=AC3:Nag+Binding+Site+For+Residue+A+2'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+1'>AC4</scene>, <scene name='pdbsite=AC5:Bgc+Binding+Site+For+Residue+B+4'>AC5</scene>, <scene name='pdbsite=AC6:Gal+Binding+Site+For+Residue+B+3'>AC6</scene>, <scene name='pdbsite=AC7:Nag+Binding+Site+For+Residue+B+2'>AC7</scene>, <scene name='pdbsite=AC8:Gal+Binding+Site+For+Residue+B+1'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Residue+A+11'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Residue+B+12'>BC1</scene>, <scene name='pdbsite=BC2:Zn+Binding+Site+For+Residue+A+13'>BC2</scene>, <scene name='pdbsite=BC3:Zn+Binding+Site+For+Residue+B+14'>BC3</scene>, <scene name='pdbsite=BC4:Zn+Binding+Site+For+Residue+B+15'>BC4</scene>, <scene name='pdbsite=BC5:Na+Binding+Site+For+Residue+B+16'>BC5</scene>, <scene name='pdbsite=BC6:Na+Binding+Site+For+Residue+A+17'>BC6</scene>, <scene name='pdbsite=BC7:Na+Binding+Site+For+Residue+A+18'>BC7</scene>, <scene name='pdbsite=BC8:Mes+Binding+Site+For+Residue+A+21'>BC8</scene> and <scene name='pdbsite=BC9:Mes+Binding+Site+For+Residue+B+22'>BC9</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8f OCA], [http://www.ebi.ac.uk/pdbsum/2z8f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z8f RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Suzuki, R.]] | [[Category: Suzuki, R.]] | ||
[[Category: Wada, J.]] | [[Category: Wada, J.]] | ||
| - | [[Category: MES]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: ZN]] | ||
[[Category: abc transporter]] | [[Category: abc transporter]] | ||
[[Category: human milk oligosacchalide]] | [[Category: human milk oligosacchalide]] | ||
| Line 34: | Line 34: | ||
[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:22 2008'' |
Revision as of 02:19, 31 March 2008
| |||||||
| , resolution 1.65Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , , , , , , , and | ||||||
| Ligands: | , , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-tetraose
Overview
A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9-6.4 and 20-22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 A for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 A for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 A resolution, respectively.
About this Structure
2Z8F is a Single protein structure of sequence from Bifidobacterium longum. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217., Wada J, Suzuki R, Fushinobu S, Kitaoka M, Wakagi T, Shoun H, Ashida H, Kumagai H, Katayama T, Yamamoto K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):751-3. Epub 2007 Aug 10. PMID:17768345
Page seeded by OCA on Mon Mar 31 05:19:22 2008
