5a1z
From Proteopedia
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| - | ''' | + | ==Cryo-EM structure of Dengue virus serotype 2 strain PVP94-07 complexed with human antibody 2D22 Fab at 37 degrees C== |
| + | <StructureSection load='5a1z' size='340' side='right' caption='[[5a1z]], [[Resolution|resolution]] 6.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5a1z]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A1Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A1Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a1z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5a1z RCSB], [http://www.ebi.ac.uk/pdbsum/5a1z PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | There are four closely-related dengue virus (DENV) serotypes. Infection with one serotype generates antibodies that may cross-react and enhance infection with other serotypes in a secondary infection. We demonstrated that DENV serotype 2 (DENV2)-specific human monoclonal antibody (HMAb) 2D22 is therapeutic in a mouse model of antibody-enhanced severe dengue disease. We determined the cryo-electron microscopy (cryo-EM) structures of HMAb 2D22 complexed with two different DENV2 strains. HMAb 2D22 binds across viral envelope (E) proteins in the dimeric structure, which probably blocks the E protein reorganization required for virus fusion. HMAb 2D22 "locks" two-thirds of or all dimers on the virus surface, depending on the strain, but neutralizes these DENV2 strains with equal potency. The epitope defined by HMAb 2D22 is a potential target for vaccines and therapeutics. | ||
| - | + | DENGUE VIRUS. Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers.,Fibriansah G, Ibarra KD, Ng TS, Smith SA, Tan JL, Lim XN, Ooi JS, Kostyuchenko VA, Wang J, de Silva AM, Harris E, Crowe JE Jr, Lok SM Science. 2015 Jul 3;349(6243):88-91. doi: 10.1126/science.aaa8651. PMID:26138979<ref>PMID:26138979</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Fibriansah, G]] | ||
[[Category: Harris, E]] | [[Category: Harris, E]] | ||
| - | [[Category: Kostyuchenko, V | + | [[Category: Ibarra, K D]] |
| - | [[Category: Lok, S | + | [[Category: Jr, J E.Crowe]] |
| - | [[Category: Ng, T | + | [[Category: Kostyuchenko, V A]] |
| - | [[Category: | + | [[Category: Lim, X N]] |
| + | [[Category: Lok, S M]] | ||
| + | [[Category: Ng, T S]] | ||
| + | [[Category: Ooi, J S.G]] | ||
| + | [[Category: Silva, A M.de]] | ||
| + | [[Category: Smith, S A]] | ||
| + | [[Category: Tan, J L]] | ||
[[Category: Wang, J]] | [[Category: Wang, J]] | ||
| - | [[Category: | + | [[Category: Cryo-em]] |
| - | [[Category: | + | [[Category: Dengue virus]] |
| - | [[Category: | + | [[Category: Human antibody]] |
| - | [[Category: | + | [[Category: Neutralization]] |
| - | [[Category: | + | [[Category: Virus]] |
| - | + | ||
Revision as of 13:23, 15 July 2015
Cryo-EM structure of Dengue virus serotype 2 strain PVP94-07 complexed with human antibody 2D22 Fab at 37 degrees C
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Categories: Homo sapiens | Fibriansah, G | Harris, E | Ibarra, K D | Jr, J E.Crowe | Kostyuchenko, V A | Lim, X N | Lok, S M | Ng, T S | Ooi, J S.G | Silva, A M.de | Smith, S A | Tan, J L | Wang, J | Cryo-em | Dengue virus | Human antibody | Neutralization | Virus
