3b6d
From Proteopedia
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|PDB= 3b6d |SIZE=350|CAPTION= <scene name='initialview01'>3b6d</scene>, resolution 1.20Å | |PDB= 3b6d |SIZE=350|CAPTION= <scene name='initialview01'>3b6d</scene>, resolution 1.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=FAE:FLAVIN-N7+PROTONATED-ADENINE+DINUCLEOTIDE'>FAE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span> |
|GENE= choA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.]) | |GENE= choA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[3b3r|3B3R]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6d OCA], [http://www.ebi.ac.uk/pdbsum/3b6d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b6d RCSB]</span> | ||
}} | }} | ||
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[[Category: Lyubimov, A Y.]] | [[Category: Lyubimov, A Y.]] | ||
[[Category: Vrielink, A.]] | [[Category: Vrielink, A.]] | ||
| - | [[Category: FAE]] | ||
| - | [[Category: SO4]] | ||
[[Category: cholesterol metabolism]] | [[Category: cholesterol metabolism]] | ||
[[Category: cholesterol oxidase]] | [[Category: cholesterol oxidase]] | ||
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[[Category: steroid metabolism]] | [[Category: steroid metabolism]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:23:24 2008'' |
Revision as of 02:23, 31 March 2008
| |||||||
| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | choA (Streptomyces sp.) | ||||||
| Activity: | Cholesterol oxidase, with EC number 1.1.3.6 | ||||||
| Related: | 3B3R
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Streptomyces Cholesterol Oxidase H447Q/E361Q mutant (1.2A)
Overview
Two high-resolution structures of a double mutant of bacterial cholesterol oxidase in the presence or absence of a ligand, glycerol, are presented, showing the trajectory of glycerol as it binds in a Michaelis complex-like position in the active site. A group of three aromatic residues forces the oxidized isoalloxazine moiety to bend along the N5-N10 axis as a response to the binding of glycerol in the active site. Movement of these aromatic residues is only observed in the glycerol-bound structure, indicating that some tuning of the FAD redox potential is caused by the formation of the Michaelis complex during regular catalysis. This structural study suggests a possible mechanism of substrate-assisted flavin activation, improves our understanding of the interplay between the enzyme, its flavin cofactor and its substrate, and is of use to the future design of effective cholesterol oxidase inhibitors.
About this Structure
3B6D is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.
Reference
Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase., Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A, Protein Sci. 2007 Dec;16(12):2647-56. PMID:18029419
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