3b6c
From Proteopedia
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|PDB= 3b6c |SIZE=350|CAPTION= <scene name='initialview01'>3b6c</scene>, resolution 2.30Å | |PDB= 3b6c |SIZE=350|CAPTION= <scene name='initialview01'>3b6c</scene>, resolution 2.30Å | ||
|SITE= <scene name='pdbsite=AC1:Sdn+Binding+Site+For+Residue+A+301'>AC1</scene>, <scene name='pdbsite=AC2:Sdn+Binding+Site+For+Residue+A+302'>AC2</scene> and <scene name='pdbsite=AC3:Sdn+Binding+Site+For+Residue+B+303'>AC3</scene> | |SITE= <scene name='pdbsite=AC1:Sdn+Binding+Site+For+Residue+A+301'>AC1</scene>, <scene name='pdbsite=AC2:Sdn+Binding+Site+For+Residue+A+302'>AC2</scene> and <scene name='pdbsite=AC3:Sdn+Binding+Site+For+Residue+B+303'>AC3</scene> | ||
| - | |LIGAND= <scene name='pdbligand=SDN:'>SDN</scene> | + | |LIGAND= <scene name='pdbligand=SDN:[(3S)-9-HYDROXY-1-METHYL-10-OXO-4,10-DIHYDRO-3H-BENZO[G]ISOCHROMEN-3-YL]ACETIC+ACID'>SDN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= actII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor]) | |GENE= actII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2opt|2OPT]], [[3b6a|3B6A]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6c OCA], [http://www.ebi.ac.uk/pdbsum/3b6c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b6c RCSB]</span> | ||
}} | }} | ||
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[[Category: Junop, M S.]] | [[Category: Junop, M S.]] | ||
[[Category: Willems, A R.]] | [[Category: Willems, A R.]] | ||
| - | [[Category: SDN]] | ||
[[Category: (s)-dnpa]] | [[Category: (s)-dnpa]] | ||
[[Category: actinorhodin]] | [[Category: actinorhodin]] | ||
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[[Category: transcriptional repressor]] | [[Category: transcriptional repressor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:23:25 2008'' |
Revision as of 02:23, 31 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , and | ||||||
| Ligands: | |||||||
| Gene: | actII (Streptomyces coelicolor) | ||||||
| Related: | 2OPT, 3B6A
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Streptomyces coelicolor TetR family protein ActR in complex with (S)-DNPA
Overview
Actinorhodin, an antibiotic produced by Streptomyces coelicolor, is exported from the cell by the ActA efflux pump. actA is divergently transcribed from actR, which encodes a TetR-like transcriptional repressor. We showed previously that ActR represses transcription by binding to an operator from the actA/actR intergenic region. Importantly, actinorhodin itself or various actinorhodin biosynthetic intermediates can cause ActR to dissociate from its operator, leading to derepression. This suggests that ActR may mediate timely self-resistance to an endogenously produced antibiotic by responding to one of its biosynthetic precursors. Here, we report the structural basis for this precursor-mediated derepression with crystal structures of homodimeric ActR by itself and in complex with either actinorhodin or the actinorhodin biosynthetic intermediate (S)-DNPA [4-dihydro-9-hydroxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-aceti c acid]. The ligand-binding tunnel in each ActR monomer has a striking hydrophilic/hydrophobic/hydrophilic arrangement of surface residues that accommodate either one hexacyclic actinorhodin molecule or two back-to-back tricyclic (S)-DNPA molecules. Moreover, our work also reveals the strongest structural evidence to date that TetR-mediated antibiotic resistance may have been acquired from an antibiotic-producer organism.
About this Structure
3B6C is a Single protein structure of sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA.
Reference
Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA., Willems AR, Tahlan K, Taguchi T, Zhang K, Lee ZZ, Ichinose K, Junop MS, Nodwell JR, J Mol Biol. 2008 Mar 7;376(5):1377-87. Epub 2008 Jan 4. PMID:18207163
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