3b7q
From Proteopedia
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|PDB= 3b7q |SIZE=350|CAPTION= <scene name='initialview01'>3b7q</scene>, resolution 2.030Å | |PDB= 3b7q |SIZE=350|CAPTION= <scene name='initialview01'>3b7q</scene>, resolution 2.030Å | ||
|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+B+311'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+A+311'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Residue+A+312'>AC3</scene>, <scene name='pdbsite=AC4:Po4+Binding+Site+For+Residue+B+312'>AC4</scene>, <scene name='pdbsite=AC5:Po4+Binding+Site+For+Residue+B+313'>AC5</scene>, <scene name='pdbsite=AC6:Po4+Binding+Site+For+Residue+A+313'>AC6</scene>, <scene name='pdbsite=AC7:6pl+Binding+Site+For+Residue+B+314'>AC7</scene>, <scene name='pdbsite=AC8:6pl+Binding+Site+For+Residue+A+314'>AC8</scene> and <scene name='pdbsite=AC9:Gol+Binding+Site+For+Residue+A+315'>AC9</scene> | |SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+B+311'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+A+311'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Residue+A+312'>AC3</scene>, <scene name='pdbsite=AC4:Po4+Binding+Site+For+Residue+B+312'>AC4</scene>, <scene name='pdbsite=AC5:Po4+Binding+Site+For+Residue+B+313'>AC5</scene>, <scene name='pdbsite=AC6:Po4+Binding+Site+For+Residue+A+313'>AC6</scene>, <scene name='pdbsite=AC7:6pl+Binding+Site+For+Residue+B+314'>AC7</scene>, <scene name='pdbsite=AC8:6pl+Binding+Site+For+Residue+A+314'>AC8</scene> and <scene name='pdbsite=AC9:Gol+Binding+Site+For+Residue+A+315'>AC9</scene> | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=6PL:(4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM+4-OXIDE'>6PL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[3b74|3B74]], [[3b7n|3B7N]], [[3b7z|3B7Z]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b7q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b7q OCA], [http://www.ebi.ac.uk/pdbsum/3b7q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b7q RCSB]</span> | ||
}} | }} | ||
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[[Category: Redinbo, M R.]] | [[Category: Redinbo, M R.]] | ||
[[Category: Schaaf, G.]] | [[Category: Schaaf, G.]] | ||
| - | [[Category: 6PL]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: PO4]] | ||
[[Category: golgi]] | [[Category: golgi]] | ||
[[Category: phosphatidylcholine]] | [[Category: phosphatidylcholine]] | ||
| Line 35: | Line 35: | ||
[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:23:52 2008'' |
Revision as of 02:23, 31 March 2008
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| , resolution 2.030Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , and | ||||||
| Ligands: | , , | ||||||
| Related: | 3B74, 3B7N, 3B7Z
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylcholine
Overview
Sec14, the major yeast phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein, regulates essential interfaces between lipid metabolism and membrane trafficking from the trans-Golgi network (TGN). How Sec14 does so remains unclear. We report that Sec14 binds PtdIns and PtdCho at distinct (but overlapping) sites, and both PtdIns- and PtdCho-binding activities are essential Sec14 activities. We further show both activities must reside within the same molecule to reconstitute a functional Sec14 and for effective Sec14-mediated regulation of phosphoinositide homeostasis in vivo. This regulation is uncoupled from PtdIns-transfer activity and argues for an interfacial presentation mode for Sec14-mediated potentiation of PtdIns kinases. Such a regulatory role for Sec14 is a primary counter to action of the Kes1 sterol-binding protein that antagonizes PtdIns 4-OH kinase activity in vivo. Collectively, these findings outline functional mechanisms for the Sec14 superfamily and reveal additional layers of complexity for regulating phosphoinositide homeostasis in eukaryotes.
About this Structure
3B7Q is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily., Schaaf G, Ortlund EA, Tyeryar KR, Mousley CJ, Ile KE, Garrett TA, Ren J, Woolls MJ, Raetz CR, Redinbo MR, Bankaitis VA, Mol Cell. 2008 Feb 1;29(2):191-206. PMID:18243114
Page seeded by OCA on Mon Mar 31 05:23:52 2008
