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4wo1

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Revision as of 02:05, 11 March 2017

Crystal structure of the DAP12 transmembrane domain in lipid cubic phase

Structural highlights

4wo1 is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	2l34, 2l35, 4wol
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[TYOBP_HUMAN] Nasu-Hakola disease. The disease is caused by mutations affecting the gene represented in this entry.

Function

[TYOBP_HUMAN] Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin.

Publication Abstract from PubMed

The membrane-spanning alpha helices of single-pass receptors play crucial roles in stabilizing oligomeric structures and transducing biochemical signals across the membrane. Probing intermolecular transmembrane interactions in single-pass receptors presents unique challenges, reflected in a gross underrepresentation of their membrane-embedded domains in structural databases. Here, we present two high-resolution structures of transmembrane assemblies from a eukaryotic single-pass protein crystallized in a lipidic membrane environment. Trimeric and tetrameric structures of the immunoreceptor signaling module DAP12, determined to 1.77-A and 2.14-A resolution, respectively, are organized by the same polar surfaces that govern intramembrane assembly with client receptors. We demonstrate that, in addition to the well-studied dimeric form, these trimeric and tetrameric structures are made in cells, and their formation is competitive with receptor association in the ER. The polar transmembrane sequences therefore act as primary determinants of oligomerization specificity through interplay between charge shielding and sequestration of polar surfaces within helix interfaces.

Transmembrane Complexes of DAP12 Crystallized in Lipid Membranes Provide Insights into Control of Oligomerization in Immunoreceptor Assembly.,Knoblich K, Park S, Lutfi M, van 't Hag L, Conn CE, Seabrook SA, Newman J, Czabotar PE, Im W, Call ME, Call MJ Cell Rep. 2015 May 26;11(8):1184-92. doi: 10.1016/j.celrep.2015.04.045. Epub 2015, May 14. PMID:25981043^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Knoblich K, Park S, Lutfi M, van 't Hag L, Conn CE, Seabrook SA, Newman J, Czabotar PE, Im W, Call ME, Call MJ. Transmembrane Complexes of DAP12 Crystallized in Lipid Membranes Provide Insights into Control of Oligomerization in Immunoreceptor Assembly. Cell Rep. 2015 May 26;11(8):1184-92. doi: 10.1016/j.celrep.2015.04.045. Epub 2015, May 14. PMID:25981043 doi:http://dx.doi.org/10.1016/j.celrep.2015.04.045

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wo1"

Categories: Call, M E | Call, M J | Knoblich, K | Alpha-helix transmembrane signaling | Signaling protein

@@ Line 1: / Line 1: @@
 ==Crystal structure of the DAP12 transmembrane domain in lipid cubic phase==
 <StructureSection load='4wo1' size='340' side='right' caption='[[4wo1]], [[Resolution|resolution]] 2.14&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2l34|2l34]], [[2l35|2l35]], [[4wol|4wol]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wo1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wo1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wo1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wo1 OCA], [http://pdbe.org/4wo1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wo1 RCSB], [http://www.ebi.ac.uk/pdbsum/4wo1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wo1 ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 19: / Line 20: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4wo1" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4wo1

From Proteopedia

Revision as of 02:05, 11 March 2017

Crystal structure of the DAP12 transmembrane domain in lipid cubic phase

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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