4rsm
From Proteopedia
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==Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. mc2 155, target efi-510970, in complex with d-threitol== | ==Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. mc2 155, target efi-510970, in complex with d-threitol== | ||
<StructureSection load='4rsm' size='340' side='right' caption='[[4rsm]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='4rsm' size='340' side='right' caption='[[4rsm]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
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<table><tr><td colspan='2'>[[4rsm]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RSM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RSM FirstGlance]. <br> | <table><tr><td colspan='2'>[[4rsm]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RSM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RSM FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3VB:D-THREITOL'>3VB</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3VB:D-THREITOL'>3VB</scene></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rsm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rsm RCSB], [http://www.ebi.ac.uk/pdbsum/4rsm PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rsm OCA], [http://pdbe.org/4rsm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rsm RCSB], [http://www.ebi.ac.uk/pdbsum/4rsm PDBsum]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discovered in microbial genome projects using the ligand specificities of the solute binding proteins (SBPs) for ABC transporters. Using differential scanning fluorimetry, we determined that the SBP for an ABC transporter encoded by the genome of Mycobacterium smegmatis is stabilized by d-threitol. Using sequence similarity networks and genome neighborhood networks to guide selection of target proteins for pathway enzymes, we applied both in vitro and in vivo experimental approaches to discover novel pathways for catabolism of d-threitol, l-threitol, and erythritol. | ||
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| + | A General Strategy for the Discovery of Metabolic Pathways: d-Threitol, l-Threitol, and Erythritol Utilization in Mycobacterium smegmatis.,Huang H, Carter MS, Vetting MW, Al-Obaidi N, Patskovsky Y, Almo SC, Gerlt JA J Am Chem Soc. 2015 Nov 25;137(46):14570-3. doi: 10.1021/jacs.5b08968. Epub 2015 , Nov 12. PMID:26560079<ref>PMID:26560079</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 4rsm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 07:37, 1 June 2016
Crystal structure of carbohydrate transporter msmeg_3599 from mycobacterium smegmatis str. mc2 155, target efi-510970, in complex with d-threitol
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Categories: Almo, S C | Attonito, J D | Bhosle, R | Chamala, S | Chowdhury, S | Efi, Enzyme Function Initiative | Gerlt, J A | Glenn, A Scott | Hillerich, B | Lafleur, J | Love, J | Morisco, L L | Obaidi, N Al | Patskovsky, Y | Siedel, R D | Toro, R | Wasserman, S R | Whalen, K L | Abc-type | Efi | Enzyme function initiative | Hydrolase | Structural genomic | Sugar transporter | Transport protein
