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3boy

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|PDB= 3boy |SIZE=350|CAPTION= <scene name='initialview01'>3boy</scene>, resolution 1.70&Aring;
|PDB= 3boy |SIZE=350|CAPTION= <scene name='initialview01'>3boy</scene>, resolution 1.70&Aring;
|SITE=
|SITE=
-
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>
+
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
|GENE= hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
 +
|DOMAIN=
 +
|RELATEDENTRY=[[1vea|1VEA]], [[1wpt|1WPT]], [[1wmq|1WMQ]], [[1wpu|1WPU]], [[1wrq|1WRQ]], [[1wpv|1WPV]]
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3boy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3boy OCA], [http://www.ebi.ac.uk/pdbsum/3boy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3boy RCSB]</span>
}}
}}
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[[Category: Shinkai, A.]]
[[Category: Shinkai, A.]]
[[Category: Yokoyama, S.]]
[[Category: Yokoyama, S.]]
-
[[Category: HIS]]
 
-
[[Category: MG]]
 
[[Category: activator]]
[[Category: activator]]
[[Category: anti-termination]]
[[Category: anti-termination]]
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[[Category: transcription/rna complex]]
[[Category: transcription/rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:00:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:27:58 2008''

Revision as of 02:27, 31 March 2008

Image:3boy.jpg


PDB ID 3boy

Drag the structure with the mouse to rotate
, resolution 1.70Å
Ligands: , , , ,
Gene: hutP (Bacillus subtilis)
Related: 1VEA, 1WPT, 1WMQ, 1WPU, 1WRQ, 1WPV


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the HutP antitermination complex bound to the HUT mRNA


Overview

HutP is an L-histidine-activated RNA binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis by binding to cis-acting regulatory sequences on the hut mRNA. The crystal structure of HutP complexed with an L-histidine analog showed a novel fold; there are four antiparallel beta strands in the central region of each monomer, with two alpha helices each on the front and back. Two HutP monomers form a dimer, and three dimers are arranged in crystallographic 3-fold symmetry to form a hexamer. A histidine analog was located in between the two monomers of HutP, with the imidazole group of L-histidine hydrogen bonded to Glu81. An activation mechanism is proposed based on the identification of key residues of HutP. The HutP binding region in hut mRNA was defined: it consists of three UAG trinucleotide motifs separated by four spacer nucleotides. Residues of HutP potentially important for RNA binding were identified.

About this Structure

3BOY is a Protein complex structure of sequences from Bacillus subtilis. This structure supersedes the now removed PDB entry 2GZT. Full crystallographic information is available from OCA.

Reference

Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:15242603

Page seeded by OCA on Mon Mar 31 05:27:58 2008

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