Subtilisin
From Proteopedia
(Difference between revisions)
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{{STRUCTURE_2z2x| PDB=2z2x | SIZE=400| SCENE=Subtilisin/Cv/1 |right|CAPTION=Subtilisin with monoisopropylphosphorylserine complex with Ca+2 ions (green) [[2z2x]] }} | {{STRUCTURE_2z2x| PDB=2z2x | SIZE=400| SCENE=Subtilisin/Cv/1 |right|CAPTION=Subtilisin with monoisopropylphosphorylserine complex with Ca+2 ions (green) [[2z2x]] }} | ||
| + | == Function == | ||
| + | [[Subtilisin]] is a serine protease. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzym<ref>PMID:4967581</ref>. See detalis in [[User:Tommie Hata/Introduction to Protein Engineering-Subtilisin]]. | ||
| - | + | *'''Selenosubtilisin''' is a semisynthetic selenoenzyme produced by chemical modification of subtilisin<ref>PMID:8385489</ref>.<br /> | |
| - | + | *'''Savinase''' is a subtilisin produced from a lentil<ref>PMID:24738747</ref>.<br /> | |
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| + | == Relevance == | ||
| + | Subtilisin is widely used commercially in detergents. | ||
== 3D Structures of Subtilisin == | == 3D Structures of Subtilisin == | ||
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**[[2wwt]], [[2x8j]] - pro-BcSub (mutant)<br /> | **[[2wwt]], [[2x8j]] - pro-BcSub (mutant)<br /> | ||
**[[3whi]] - pro-BcSub (mutant)<br /> | **[[3whi]] - pro-BcSub (mutant)<br /> | ||
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| - | * Mutant unautoprocessed subtilisin | ||
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| - | **[[2zwo]], [[2zwp]] – pro-TKSub | ||
* Mature subtilisin complexed with its propeptide | * Mature subtilisin complexed with its propeptide | ||
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**[[3bx1]], [[1svn]], [[4cfy]], [[4cfz]], [[4cg0]] – Sav – ''Bacillus lentus'' | **[[3bx1]], [[1svn]], [[4cfy]], [[4cfz]], [[4cg0]] – Sav – ''Bacillus lentus'' | ||
}} | }} | ||
| + | == References == | ||
| + | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 06:53, 4 September 2016
Contents |
Function
Subtilisin is a serine protease. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzym[1]. See detalis in User:Tommie Hata/Introduction to Protein Engineering-Subtilisin.
- Selenosubtilisin is a semisynthetic selenoenzyme produced by chemical modification of subtilisin[2].
- Savinase is a subtilisin produced from a lentil[3].
Relevance
Subtilisin is widely used commercially in detergents.
3D Structures of Subtilisin
Updated on 04-September-2016
References
- ↑ Smith EL, DeLange RJ, Evans WH, Landon M, Markland FS. Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships. J Biol Chem. 1968 May 10;243(9):2184-91. PMID:4967581
- ↑ Bell IM, Fisher ML, Wu ZP, Hilvert D. Kinetic studies on the peroxidase activity of selenosubtilisin. Biochemistry. 1993 Apr 13;32(14):3754-62. PMID:8385489
- ↑ Garcia-Mora P, Penas E, Frias J, Martinez-Villaluenga C. Savinase, the most suitable enzyme for releasing peptides from lentil (Lens culinaris var. Castellana) protein concentrates with multifunctional properties. J Agric Food Chem. 2014 May 7;62(18):4166-74. doi: 10.1021/jf500849u. Epub 2014, Apr 28. PMID:24738747 doi:http://dx.doi.org/10.1021/jf500849u
