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4d0g

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Revision as of 13:20, 10 May 2017

Structure of Rab14 in complex with Rab-Coupling Protein (RCP)

Structural highlights

4d0g is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RAB14_HUMAN] Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes (By similarity). Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion.^[1] [RFIP1_HUMAN] A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

Rab GTPases recruit effector proteins, via their GTP-dependent switch regions, to distinct sub-cellular compartments. Rab11 and Rab25 are closely related small GTPases that bind to common effectors termed the Rab11-Family of Interacting Proteins (FIPs). The FIPs are organized into two subclasses (class I and class II) based on sequence and domain organization, and both subclasses contain a highly conserved Rab-binding domain (RBD) at their C-termini. Yeast two-hybrid and biochemical studies have revealed that the more distantly related Rab14 also interacts with class I FIPs. Here, we perform detailed structural, thermodynamic and cellular analyses of the interactions between Rab14 and one of the class I FIPs - the Rab Coupling Protein (RCP) - to clarify the molecular aspects of the interaction. We find that Rab14 indeed binds to RCP, albeit with reduced affinity relative to conventional Rab11:FIP and Rab25:FIP complexes. However, in vivo, Rab11 recruits RCP onto biological membranes. Furthermore, biophysical analyses reveal a non-canonical 1:2 stoichiometry between Rab14:RCP in dilute solutions, in contrast to Rab11/25 complexes. The structure of Rab14:RCP reveals that Rab14 interacts with the canonical RBD, and also provides insight into the unusual properties of the complex. Finally, we show that both the Rab Coupling Protein and Rab14 function in neuritogenesis.

Structure-Function Analyses of the Interactions Between Rab11 and Rab14 Small GTPases with their Shared Effector Rab Coupling Protein (RCP).,Lall P, Lindsay A, Hanscom S, Kecman T, Taglauer ES, McVeigh UM, Franklin E, McCaffrey MW, Khan AR J Biol Chem. 2015 May 31. pii: jbc.M114.612366. PMID:26032412^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Linford A, Yoshimura S, Nunes Bastos R, Langemeyer L, Gerondopoulos A, Rigden DJ, Barr FA. Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability in migrating cells. Dev Cell. 2012 May 15;22(5):952-66. doi: 10.1016/j.devcel.2012.04.010. PMID:22595670 doi:http://dx.doi.org/10.1016/j.devcel.2012.04.010
↑ Lindsay AJ, Hendrick AG, Cantalupo G, Senic-Matuglia F, Goud B, Bucci C, McCaffrey MW. Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein. J Biol Chem. 2002 Apr 5;277(14):12190-9. Epub 2002 Jan 10. PMID:11786538 doi:http://dx.doi.org/10.1074/jbc.M108665200
↑ Peden AA, Schonteich E, Chun J, Junutula JR, Scheller RH, Prekeris R. The RCP-Rab11 complex regulates endocytic protein sorting. Mol Biol Cell. 2004 Aug;15(8):3530-41. Epub 2004 Jun 4. PMID:15181150 doi:http://dx.doi.org/10.1091/mbc.E03-12-0918
↑ Damiani MT, Pavarotti M, Leiva N, Lindsay AJ, McCaffrey MW, Colombo MI. Rab coupling protein associates with phagosomes and regulates recycling from the phagosomal compartment. Traffic. 2004 Oct;5(10):785-97. PMID:15355514 doi:http://dx.doi.org/10.1111/j.1600-0854.2004.00220.x
↑ Jin M, Goldenring JR. The Rab11-FIP1/RCP gene codes for multiple protein transcripts related to the plasma membrane recycling system. Biochim Biophys Acta. 2006 Jun;1759(6):281-95. Epub 2006 Jun 27. PMID:16920206 doi:http://dx.doi.org/S0167-4781(06)00093-5
↑ Lall P, Lindsay A, Hanscom S, Kecman T, Taglauer ES, McVeigh UM, Franklin E, McCaffrey MW, Khan AR. Structure-Function Analyses of the Interactions Between Rab11 and Rab14 Small GTPases with their Shared Effector Rab Coupling Protein (RCP). J Biol Chem. 2015 May 31. pii: jbc.M114.612366. PMID:26032412 doi:http://dx.doi.org/10.1074/jbc.M114.612366

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4d0g"

@@ Line 1: / Line 1: @@
 ==Structure of Rab14 in complex with Rab-Coupling Protein (RCP)==
 <StructureSection load='4d0g' size='340' side='right' caption='[[4d0g]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4d0g]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D0G FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4d0g RCSB], [http://www.ebi.ac.uk/pdbsum/4d0g PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d0g OCA], [http://pdbe.org/4d0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d0g RCSB], [http://www.ebi.ac.uk/pdbsum/4d0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4d0g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4d0g" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4d0g

From Proteopedia

Revision as of 13:20, 10 May 2017

Structure of Rab14 in complex with Rab-Coupling Protein (RCP)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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