3c4t
From Proteopedia
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|PDB= 3c4t |SIZE=350|CAPTION= <scene name='initialview01'>3c4t</scene>, resolution 2.80Å | |PDB= 3c4t |SIZE=350|CAPTION= <scene name='initialview01'>3c4t</scene>, resolution 2.80Å | ||
|SITE= <scene name='pdbsite=AC1:Cd+Binding+Site+For+Residue+A+2'>AC1</scene> and <scene name='pdbsite=AC2:Cd+Binding+Site+For+Residue+A+3'>AC2</scene> | |SITE= <scene name='pdbsite=AC1:Cd+Binding+Site+For+Residue+A+2'>AC1</scene> and <scene name='pdbsite=AC2:Cd+Binding+Site+For+Residue+A+3'>AC2</scene> | ||
| - | |LIGAND= <scene name='pdbligand=CD:CADMIUM ION'>CD</scene> | + | |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Dicer1, Dicer, Mdcr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |GENE= Dicer1, Dicer, Mdcr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[3c4b|3C4B]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c4t OCA], [http://www.ebi.ac.uk/pdbsum/3c4t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3c4t RCSB]</span> | ||
}} | }} | ||
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[[Category: Stroud, R M.]] | [[Category: Stroud, R M.]] | ||
[[Category: Tjhen, R J.]] | [[Category: Tjhen, R J.]] | ||
| - | [[Category: CD]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: endonuclease]] | [[Category: endonuclease]] | ||
| Line 39: | Line 41: | ||
[[Category: rnase]] | [[Category: rnase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:30:37 2008'' |
Revision as of 02:30, 31 March 2008
| |||||||
| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | , | ||||||
| Gene: | Dicer1, Dicer, Mdcr (Mus musculus) | ||||||
| Related: | 3C4B
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of RNaseIIIb and dsRNA binding domains of mouse Dicer
Overview
Dicer, an RNase III enzyme, initiates RNA interference by processing precursor dsRNAs into mature microRNAs and small-interfering RNAs. It is also involved in loading and activation of the RNA-induced silencing complex. Here, we report the crystal structures of a catalytically active fragment of mouse Dicer, containing the RNase IIIb and dsRNA binding domains, in its apo and Cd(2+)-bound forms, at 1.68- and 2.8-A resolution, respectively. Models of this structure with dsRNA reveal that a lysine residue, highly conserved in Dicer RNase IIIa and IIIb domains and in Drosha RNase IIIb domains, has the potential to participate in the phosphodiester bond cleavage reaction by stabilizing the transition state and leaving group of the scissile bond. Mutational and enzymatic assays confirm the importance of this lysine in dsRNA cleavage, suggesting that this lysine represents a conserved catalytic residue of Dicers. The structures also reveals a approximately 45-aa region within the RNase IIIb domain that harbors an alpha-helix at the N-terminal half and a flexible loop at the C-terminal half, features not present in previously reported structures of homologous RNase III domains from either bacterial RNase III enzymes or Giardia Dicer. N-terminal residues of this alpha-helix have the potential to engage in minor groove interaction with dsRNA substrates.
About this Structure
3C4T is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage., Du Z, Lee JK, Tjhen R, Stroud RM, James TL, Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2391-6. Epub 2008 Feb 11. PMID:18268334
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