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2mqk

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Revision as of 13:46, 5 April 2017

Solution structure of N terminal domain of the MuB AAA+ ATPase

Structural highlights

2mqk is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	Adenosinetriphosphatase, with EC number 3.6.1.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TARGB_BPMU] Selects the target DNA sites for transposition. Recruits DDE-recombinase A to the target sites and catalytically activates it. Displays non-specific DNA-binding properties. Polymerizes as helical filaments around the DNA. Coating of the DNA by the target DNA activator B might play a role in favoring target-primed replication over integration. Prevents self-integration into an integrated copy of the viral genome. This mechanism is called target immunity and is achieved by two mechanisms: first, the target DNA activator B dissociates from the viral genome ends upon interaction in cis with DDE-recombinase A, which makes the viral genome ends a poor target for new insertions. Second, the interior of the viral genome may also ne protected from integration events by the target DNA activator B being strongly bound throughout the whole viral genome.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8]

References

↑ Roldan LA, Baker TA. Differential role of the Mu B protein in phage Mu integration vs. replication: mechanistic insights into two transposition pathways. Mol Microbiol. 2001 Apr;40(1):141-55. PMID:11298282
↑ Goldhaber-Gordon I, Early MH, Baker TA. MuA transposase separates DNA sequence recognition from catalysis. Biochemistry. 2003 Dec 16;42(49):14633-42. PMID:14661976 doi:http://dx.doi.org/10.1021/bi035360o
↑ Baker TA, Mizuuchi M, Mizuuchi K. MuB protein allosterically activates strand transfer by the transposase of phage Mu. Cell. 1991 Jun 14;65(6):1003-13. PMID:1646076
↑ Tan X, Mizuuchi M, Mizuuchi K. DNA transposition target immunity and the determinants of the MuB distribution patterns on DNA. Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13925-9. Epub 2007 Aug 20. PMID:17709741 doi:http://dx.doi.org/10.1073/pnas.0706564104
↑ Lemberg KM, Schweidenback CT, Baker TA. The dynamic Mu transpososome: MuB activation prevents disintegration. J Mol Biol. 2007 Dec 14;374(5):1158-71. Epub 2007 Oct 3. PMID:17988683 doi:http://dx.doi.org/10.1016/j.jmb.2007.09.079
↑ Ge J, Lou Z, Harshey RM. Immunity of replicating Mu to self-integration: a novel mechanism employing MuB protein. Mob DNA. 2010 Feb 1;1(1):8. doi: 10.1186/1759-8753-1-8. PMID:20226074 doi:http://dx.doi.org/10.1186/1759-8753-1-8
↑ Mizuno N, Dramicanin M, Mizuuchi M, Adam J, Wang Y, Han YW, Yang W, Steven AC, Mizuuchi K, Ramon-Maiques S. MuB is an AAA+ ATPase that forms helical filaments to control target selection for DNA transposition. Proc Natl Acad Sci U S A. 2013 Jun 17. PMID:23776210 doi:10.1073/pnas.1309499110
↑ Dramicanin M, Ramon-Maiques S. MuB gives a new twist to target DNA selection. Mob Genet Elements. 2013 Sep 1;3(5):e27515. Epub 2013 Dec 12. PMID:24478936 doi:http://dx.doi.org/10.4161/mge.27515

1 Structural highlights
2 Function
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mqk"

@@ Line 1: / Line 1: @@
 ==Solution structure of N terminal domain of the MuB AAA+ ATPase==
 <StructureSection load='2mqk' size='340' side='right' caption='[[2mqk]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2mqk]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MQK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MQK FirstGlance]. <br>
 </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mqk RCSB], [http://www.ebi.ac.uk/pdbsum/2mqk PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mqk OCA], [http://pdbe.org/2mqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mqk RCSB], [http://www.ebi.ac.uk/pdbsum/2mqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mqk ProSAT]</span></td></tr>
 </table>
 == Function ==

2mqk

From Proteopedia

Revision as of 13:46, 5 April 2017

Solution structure of N terminal domain of the MuB AAA+ ATPase

Structural highlights

Function

References

Contents

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