5c5v
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Recombinant Inorganic Pyrophosphatase from T brucei brucei== |
| + | <StructureSection load='5c5v' size='340' side='right' caption='[[5c5v]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5c5v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C5V FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PN:IMIDODIPHOSPHORIC+ACID'>2PN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c5v OCA], [http://pdbe.org/5c5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c5v RCSB], [http://www.ebi.ac.uk/pdbsum/5c5v PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Trypanosoma brucei (T. brucei) is responsible for the fatal human disease called African trypanosomiasis or sleeping sickness. The causative parasite Trypanosoma encodes soluble versions of inorganic pyrophosphatases (PPase), also called vacuolar soluble proteins (VSPs), which are localized to its acidocalcisomes. Latter are acidic membrane enclosed organelles rich in polyphosphate chains and divalent cations whose significance in these parasites remains unclear. We here report the crystal structure of T. brucei brucei acidocalcisomal PPases in a ternary complex with Mg2+ and imidodiphosphate. The crystal structure reveals a novel structural architecture distinct from known class I PPases in its tetrameric oligomeric state in which a fused EF-hand domain arranges around the catalytic PPase domain. This unprecedented assembly evident from TbbVSP1 crystal structure is further confirmed by small angle X-ray scattering (SAXS) and electron microscopy data. Solution scattering data suggests structural flexibility in EF-hand domains indicative of conformational plasticity within TbbVSP1. | ||
| - | + | Structural and Functional highlights of Vacuolar Soluble Protein 1 from pathogen T. brucei. brucei.,Jamwal A, Round AR, Bannwarth L, Bryan CV, Belrahli H, Yogavel M, Sharma A J Biol Chem. 2015 Oct 22. pii: jbc.M115.674176. PMID:26494625<ref>PMID:26494625</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5c5v" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Jamwal, A]] | [[Category: Jamwal, A]] | ||
[[Category: Sharma, A]] | [[Category: Sharma, A]] | ||
| + | [[Category: Yogavel, M]] | ||
| + | [[Category: Ca binding protein]] | ||
| + | [[Category: Ef-hand]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Ppase domain]] | ||
Revision as of 07:25, 9 December 2015
Recombinant Inorganic Pyrophosphatase from T brucei brucei
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