7msf

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|PDB= 7msf |SIZE=350|CAPTION= <scene name='initialview01'>7msf</scene>, resolution 2.8&Aring;
|PDB= 7msf |SIZE=350|CAPTION= <scene name='initialview01'>7msf</scene>, resolution 2.8&Aring;
|SITE=
|SITE=
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|LIGAND=
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= COAT PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Enterobacterio phage MS2])
|GENE= COAT PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Enterobacterio phage MS2])
 +
|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7msf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7msf OCA], [http://www.ebi.ac.uk/pdbsum/7msf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=7msf RCSB]</span>
}}
}}
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[[Category: rna-protein complex]]
[[Category: rna-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:14:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:44:31 2008''

Revision as of 02:44, 31 March 2008

Image:7msf.gif


PDB ID 7msf

Drag the structure with the mouse to rotate
, resolution 2.8Å
Ligands: , , ,
Gene: COAT PROTEIN (Enterobacterio phage MS2)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



MS2 PROTEIN CAPSID/RNA COMPLEX


Overview

We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.

About this Structure

7MSF is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.

Reference

Crystal structures of a series of RNA aptamers complexed to the same protein target., Rowsell S, Stonehouse NJ, Convery MA, Adams CJ, Ellington AD, Hirao I, Peabody DS, Stockley PG, Phillips SE, Nat Struct Biol. 1998 Nov;5(11):970-5. PMID:9808042

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