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4tl8

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Revision as of 13:22, 10 May 2017

Crystal structure of N-terminal C1 domain of KaiC

Structural highlights

4tl8 is a 6 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	4tl6, 4tl7, 4tl9, 4tla, 4tlb, 4tlc, 4tld, 4tle
Activity:	Non-specific serine/threonine protein kinase, with EC number 2.7.11.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KAIC_SYNE7] Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction.^[1] ^[2]

Publication Abstract from PubMed

Circadian clocks generate slow and ordered cellular dynamics, but consist of fast-moving bio-macromolecules; consequently, the origins of the overall slowness remain unclear. We identified the adenosine triphosphate catalytic region (ATPase) in the N-terminal half of the clock protein KaiC as the minimal pacemaker that controls the in vivo frequency of the cyanobacterial clock. Crystal structures of the ATPase revealed that the slowness of this ATPase arises from sequestration of a lytic water molecule in an unfavorable position and coupling of ATP hydrolysis to a peptide isomerization with high activation energy. The slow ATPase is coupled with another ATPase catalyzing autodephosphorylation in the C-terminal half of KaiC, yielding the circadian response frequency of intermolecular interactions with other clock-related proteins that influences the transcription and translation cycle.

Atomic-scale origins of slowness in the cyanobacterial circadian clock.,Abe J, Hiyama TB, Mukaiyama A, Son S, Mori T, Saito S, Osako M, Wolanin J, Yamashita E, Kondo T, Akiyama S Science. 2015 Jun 25. pii: 1261040. PMID:26113637^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ishiura M, Kutsuna S, Aoki S, Iwasaki H, Andersson CR, Tanabe A, Golden SS, Johnson CH, Kondo T. Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria. Science. 1998 Sep 4;281(5382):1519-23. PMID:9727980
↑ Nakahira Y, Katayama M, Miyashita H, Kutsuna S, Iwasaki H, Oyama T, Kondo T. Global gene repression by KaiC as a master process of prokaryotic circadian system. Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):881-5. Epub 2004 Jan 6. PMID:14709675 doi:10.1073/pnas.0307411100
↑ Abe J, Hiyama TB, Mukaiyama A, Son S, Mori T, Saito S, Osako M, Wolanin J, Yamashita E, Kondo T, Akiyama S. Atomic-scale origins of slowness in the cyanobacterial circadian clock. Science. 2015 Jun 25. pii: 1261040. PMID:26113637 doi:http://dx.doi.org/10.1126/science.1261040

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4tl8"

@@ Line 1: / Line 1: @@
 ==Crystal structure of N-terminal C1 domain of KaiC==
 <StructureSection load='4tl8' size='340' side='right' caption='[[4tl8]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4tl6|4tl6]], [[4tl7|4tl7]], [[4tl9|4tl9]], [[4tla|4tla]], [[4tlb|4tlb]], [[4tlc|4tlc]], [[4tld|4tld]], [[4tle|4tle]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tl8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tl8 RCSB], [http://www.ebi.ac.uk/pdbsum/4tl8 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tl8 OCA], [http://pdbe.org/4tl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tl8 RCSB], [http://www.ebi.ac.uk/pdbsum/4tl8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tl8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4tl8" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Circadian clock protein|Circadian clock protein]]
 == References ==
 <references/>

4tl8

From Proteopedia

Revision as of 13:22, 10 May 2017

Crystal structure of N-terminal C1 domain of KaiC

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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