8psh
From Proteopedia
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|PDB= 8psh |SIZE=350|CAPTION= <scene name='initialview01'>8psh</scene> | |PDB= 8psh |SIZE=350|CAPTION= <scene name='initialview01'>8psh</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=G:GUANOSINE-5 | + | |LIGAND= <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
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[[Category: thionucleotide]] | [[Category: thionucleotide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 16:06:59 2008'' |
Revision as of 14:06, 23 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE
Overview
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
About this Structure
8PSH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216
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