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4cmy

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Revision as of 16:09, 17 May 2017

Chlorobium tepidum Ferritin

Structural highlights

4cmy is a 24 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q8KBP5_CHLTE] Iron storage protein.[RuleBase:RU361145]

Publication Abstract from PubMed

Ferritins are ubiquitous iron-storage proteins found in all kingdoms of life. They share a common architecture made of 24 subunits of five alpha-helices. The recombinant Chlorobium tepidum ferritin (rCtFtn) is a structurally interesting protein since sequence alignments with other ferritins show that this protein has a significantly extended C-terminus, which possesses 12 histidine residues as well as several aspartate and glutamic acid residues that are potential metal ion binding residues. We show that the macromolecular assembly of rCtFtn exhibits a cage-like hollow shell consisting of 24 monomers that are related by 4-3-2 symmetry; similar to the assembly of other ferritins. In all ferritins of known structure the short fifth alpha-helix adopts an acute angle with respect to the four-helix bundle. However, the crystal structure of the rCtFtn presented here shows that this helix adopts a new conformation defining a new assembly of the 4-fold channel of rCtFtn. This conformation allows the arrangement of the C-terminal region into the inner cavity of the protein shell. Furthermore, two Fe(III) ions were found in each ferroxidase center of rCtFtn, with an average FeA-FeB distance of 3 A; corresponding to a diferric mu-oxo/hydroxo species. This is the first ferritin crystal structure with an isolated di-iron center in an iron-storage ferritin. The crystal structure of rCtFtn and the biochemical results presented here, suggests that rCtFtn presents similar biochemical properties reported for other members of this protein family albeit with distinct structural plasticity.

The crystal structure of ferritin from Chlorobium tepidum reveals a new conformation of the 4-fold channel for this protein family.,Arenas-Salinas M, Townsend PD, Brito C, Marquez V, Marabolli V, Gonzalez-Nilo F, Matias C, Watt RK, Lopez-Castro JD, Dominguez-Vera J, Pohl E, Yevenes A Biochimie. 2014 Nov;106:39-47. doi: 10.1016/j.biochi.2014.07.019. Epub 2014 Jul, 28. PMID:25079050^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Arenas-Salinas M, Townsend PD, Brito C, Marquez V, Marabolli V, Gonzalez-Nilo F, Matias C, Watt RK, Lopez-Castro JD, Dominguez-Vera J, Pohl E, Yevenes A. The crystal structure of ferritin from Chlorobium tepidum reveals a new conformation of the 4-fold channel for this protein family. Biochimie. 2014 Nov;106:39-47. doi: 10.1016/j.biochi.2014.07.019. Epub 2014 Jul, 28. PMID:25079050 doi:http://dx.doi.org/10.1016/j.biochi.2014.07.019

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4cmy"

@@ Line 1: / Line 1: @@
 ==Chlorobium tepidum Ferritin==
 <StructureSection load='4cmy' size='340' side='right' caption='[[4cmy]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4cmy]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CMY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CMY FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cmy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4cmy RCSB], [http://www.ebi.ac.uk/pdbsum/4cmy PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cmy OCA], [http://pdbe.org/4cmy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cmy RCSB], [http://www.ebi.ac.uk/pdbsum/4cmy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cmy ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4cmy" style="background-color:#fffaf0;"></div>
 ==See Also==

4cmy

From Proteopedia

Revision as of 16:09, 17 May 2017

Chlorobium tepidum Ferritin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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