4v6u

Structural highlights

Function

[RL3_PYRFU] One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit. [RL18_PYRFU] This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. [RS14Z_PYRFU] Binds 16S rRNA, required for the assembly of 30S particles. [RS5_PYRFU] With S4 and S12 plays an important role in translational accuracy.[HAMAP-Rule:MF_01307] [RL7A_PYRFU] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA, box C/D and box C'/D' sRNAs (By similarity). [RS4_PYRFU] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit. With S5 and S12 plays an important role in translational accuracy. [RL15_PYRFU] Binds to the 23S rRNA. [RL14_PYRFU] Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome. [RS10_PYRFU] Involved in the binding of tRNA to the ribosomes. [RL24E_PYRFU] Binds to the 23S rRNA. [RL37_PYRFU] Binds to the 23S rRNA. [RS12_PYRFU] With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits. [RL2_PYRFU] One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. [RL22_PYRFU] This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome. [RL11_PYRFU] Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. [RL37A_PYRFU] Binds to the 23S rRNA. [RL10_PYRFU] Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors. [RL6_PYRFU] This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center. [RS13_PYRFU] Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. [Q8U0T4_PYRFU] May be involved in maturation of the 30S ribosomal subunit.[HAMAP-Rule:MF_01474] [RS7_PYRFU] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center. [RL5_PYRFU] This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs. [RS11_PYRFU] Located on the platform of the 30S subunit. [RL19E_PYRFU] Binds to the 23S rRNA.[HAMAP-Rule:MF_01475] [RL4_PYRFU] One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome. Forms part of the polypeptide exit tunnel. [RL23_PYRFU] Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.[HAMAP-Rule:MF_01369] [RL1_PYRFU] Binds directly to 23S rRNA. Probably involved in E site tRNA release. Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA. [RS19_PYRFU] Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. [RS3_PYRFU] Binds the lower part of the 30S subunit head. [RS8_PYRFU] One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit. [RL44E_PYRFU] Binds to the 23S rRNA. [RL13_PYRFU] This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly. [RS17_PYRFU] One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA. [RL24_PYRFU] One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.[HAMAP-Rule:MF_01326] Located at the polypeptide exit tunnel on the outside of the subunit.[HAMAP-Rule:MF_01326]

See Also

• Ribosome 3D structures
• TRNA

References

1. ↑ Armache JP, Anger AM, Marquez V, Franckenberg S, Frohlich T, Villa E, Berninghausen O, Thomm M, Arnold GJ, Beckmann R, Wilson DN. Promiscuous behaviour of archaeal ribosomal proteins: Implications for eukaryotic ribosome evolution. Nucleic Acids Res. 2012 Dec 6. PMID:23222135 doi:http://dx.doi.org/10.1093/nar/gks1259