5bob
From Proteopedia
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| - | ''' | + | ==Crystal Structure of the Meningitis Pathogen Streptococcus suis adhesion Fhb== |
| + | <StructureSection load='5bob' size='340' side='right' caption='[[5bob]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5bob]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BOB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BOB FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5boa|5boa]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bob OCA], [http://pdbe.org/5bob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bob RCSB], [http://www.ebi.ac.uk/pdbsum/5bob PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fhb is a surface virulence protein from Streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor H to inactivate C3b deposited on bacterial surface in blood. Here we successfully expressed and purified the N terminal domain of Fhb (N-Fhb) and obtained crystals of the N-Fhb by sitting-drop vapor diffusion method with a resolution of 1.50 A. The crystals belong to space group C2 with unit cell parameters a = 127.1 A, b = 77.3 A, c = 131.6 A, alpha = 90 degrees , beta = 115.9 degrees , gamma = 90 degrees . The structure of N-Fhb was determined by SAD method and the core structure of N-Fhb is a beta sandwich. We speculated that binding of Fhb to human factor H may be mainly mediated by surface amino acids with negative charges. | ||
| - | + | Expression, purification, crystallization and structure determination of the N terminal domain of Fhb, a factor H binding protein from Streptococcus suis.,Zhang C, Yu Y, Yang M, Jiang Y Biochem Biophys Res Commun. 2015 Oct 23;466(3):413-7. doi:, 10.1016/j.bbrc.2015.09.040. Epub 2015 Sep 10. PMID:26365348<ref>PMID:26365348</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5bob" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Jiang, Y]] | [[Category: Jiang, Y]] | ||
[[Category: Yu, Y]] | [[Category: Yu, Y]] | ||
[[Category: Zhang, C]] | [[Category: Zhang, C]] | ||
| + | [[Category: Beta sandwich core]] | ||
| + | [[Category: Translation]] | ||
Revision as of 18:58, 1 December 2015
Crystal Structure of the Meningitis Pathogen Streptococcus suis adhesion Fhb
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