1jof
From Proteopedia
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|PDB= 1jof |SIZE=350|CAPTION= <scene name='initialview01'>1jof</scene>, resolution 2.5Å | |PDB= 1jof |SIZE=350|CAPTION= <scene name='initialview01'>1jof</scene>, resolution 2.5Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PIN:PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC+ACID)'>PIN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Carboxy-cis,cis-muconate_cyclase Carboxy-cis,cis-muconate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.5 5.5.1.5] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxy-cis,cis-muconate_cyclase Carboxy-cis,cis-muconate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.5 5.5.1.5] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1muc|1MUC]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jof OCA], [http://www.ebi.ac.uk/pdbsum/1jof PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jof RCSB]</span> | ||
}} | }} | ||
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[[Category: Merckel, M C.]] | [[Category: Merckel, M C.]] | ||
[[Category: Thompson, A.]] | [[Category: Thompson, A.]] | ||
| - | [[Category: BME]] | ||
| - | [[Category: PIN]] | ||
| - | [[Category: SO4]] | ||
[[Category: beta-propeller]] | [[Category: beta-propeller]] | ||
[[Category: homotetramer]] | [[Category: homotetramer]] | ||
[[Category: semet-protein]] | [[Category: semet-protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:36:33 2008'' |
Revision as of 18:36, 30 March 2008
| |||||||
| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Activity: | Carboxy-cis,cis-muconate cyclase, with EC number 5.5.1.5 | ||||||
| Related: | 1MUC
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme
Overview
Muconate lactonizing enzymes (MLEs) convert cis,cis-muconates to muconolactones in microbes as part of the beta-ketoadipate pathway; some also dehalogenate muconate derivatives of xenobiotic haloaromatics. There are three different MLE classes unrelated by evolution. We present the X-ray structure of a eukaryotic MLE, Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme (NcCMLE) at 2.5 A resolution, with a seven-bladed beta propeller fold. It is related neither to bacterial MLEs nor to other beta propeller enzymes, but is structurally similar to the G protein beta subunit. It reveals a novel metal-independent cycloisomerase motif unlike the bacterial metal cofactor MLEs. Together, the bacterial MLEs and NcCMLE structures comprise a striking structural example of functional convergence in enzymes for 1,2-addition-elimination of carboxylic acids. NcCMLE and bacterial MLEs may enhance the reaction rate differently: the former by electrophilic catalysis and the latter by electrostatic stabilization of the enolate.
About this Structure
1JOF is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.
Reference
The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase., Kajander T, Merckel MC, Thompson A, Deacon AM, Mazur P, Kozarich JW, Goldman A, Structure. 2002 Apr;10(4):483-92. PMID:11937053
Page seeded by OCA on Sun Mar 30 21:36:33 2008
