1l2t
From Proteopedia
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|PDB= 1l2t |SIZE=350|CAPTION= <scene name='initialview01'>1l2t</scene>, resolution 1.90Å | |PDB= 1l2t |SIZE=350|CAPTION= <scene name='initialview01'>1l2t</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2t OCA], [http://www.ebi.ac.uk/pdbsum/1l2t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1l2t RCSB]</span> | ||
}} | }} | ||
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[[Category: Rosen, J.]] | [[Category: Rosen, J.]] | ||
[[Category: Smith, P C.]] | [[Category: Smith, P C.]] | ||
| - | [[Category: ATP]] | ||
| - | [[Category: IPA]] | ||
| - | [[Category: NA]] | ||
[[Category: abc transporter]] | [[Category: abc transporter]] | ||
[[Category: atpase]] | [[Category: atpase]] | ||
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[[Category: walker-a]] | [[Category: walker-a]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:57:09 2008'' |
Revision as of 18:57, 30 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Dimeric Structure of MJ0796, a Bacterial ABC Transporter Cassette
Overview
It has been proposed that the reaction cycle of ATP binding cassette (ABC) transporters is driven by dimerization of their ABC motor domains upon binding ATP at their mutual interface. However, no such ATP sandwich complex has been observed for an ABC from an ABC transporter. In this paper, we report the crystal structure of a stable dimer formed by the E171Q mutant of the MJ0796 ABC, which is hydrolytically inactive due to mutation of the catalytic base. The structure shows a symmetrical dimer in which two ATP molecules are each sandwiched between the Walker A motif in one subunit and the LSGGQ signature motif in the other subunit. These results establish the stereochemical basis of the power stroke of ABC transporter pumps.
About this Structure
1L2T is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer., Smith PC, Karpowich N, Millen L, Moody JE, Rosen J, Thomas PJ, Hunt JF, Mol Cell. 2002 Jul;10(1):139-49. PMID:12150914
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