1ltq
From Proteopedia
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|PDB= 1ltq |SIZE=350|CAPTION= <scene name='initialview01'>1ltq</scene>, resolution 2.33Å | |PDB= 1ltq |SIZE=350|CAPTION= <scene name='initialview01'>1ltq</scene>, resolution 2.33Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Polynucleotide_5'-hydroxy-kinase Polynucleotide 5'-hydroxy-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.78 2.7.1.78] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ltq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ltq OCA], [http://www.ebi.ac.uk/pdbsum/1ltq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ltq RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LTQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1LTQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTQ OCA]. |
==Reference== | ==Reference== | ||
Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase., Galburt EA, Pelletier J, Wilson G, Stoddard BL, Structure. 2002 Sep;10(9):1249-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12220496 12220496] | Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase., Galburt EA, Pelletier J, Wilson G, Stoddard BL, Structure. 2002 Sep;10(9):1249-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12220496 12220496] | ||
| - | [[Category: | + | [[Category: Enterobacteria phage t4]] |
[[Category: Polynucleotide 5'-hydroxy-kinase]] | [[Category: Polynucleotide 5'-hydroxy-kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
[[Category: Wilson, G.]] | [[Category: Wilson, G.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: DMS]] | ||
[[Category: alpha/beta]] | [[Category: alpha/beta]] | ||
[[Category: kinase]] | [[Category: kinase]] | ||
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[[Category: phosphatase]] | [[Category: phosphatase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:13 2008'' |
Revision as of 19:07, 30 March 2008
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| , resolution 2.33Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Polynucleotide 5'-hydroxy-kinase, with EC number 2.7.1.78 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF T4 POLYNUCLEOTIDE KINASE
Overview
T4 phage polynucleotide kinase (PNK) was identified over 35 years ago and has become a staple reagent for molecular biologists. The enzyme displays 5'-hydroxyl kinase, 3'-phosphatase, and 2',3'-cyclic phosphodiesterase activities against a wide range of substrates. These activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase. DNA repair enzymes that share conserved motifs with PNK have been identified in eukaryotes. PNK contains two functionally distinct structural domains and forms a homotetramer. The C-terminal phosphatase domain is homologous to the L-2-haloacid dehalogenase family and the N-terminal kinase domain is homologous to adenylate kinase. The active sites have been characterized through structural homology analyses and visualization of bound substrate.
About this Structure
1LTQ is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Structure of a tRNA repair enzyme and molecular biology workhorse: T4 polynucleotide kinase., Galburt EA, Pelletier J, Wilson G, Stoddard BL, Structure. 2002 Sep;10(9):1249-60. PMID:12220496
Page seeded by OCA on Sun Mar 30 22:07:13 2008
