1p7c
From Proteopedia
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|PDB= 1p7c |SIZE=350|CAPTION= <scene name='initialview01'>1p7c</scene>, resolution 2.10Å | |PDB= 1p7c |SIZE=350|CAPTION= <scene name='initialview01'>1p7c</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=T5A:P1-(5'-ADENOSYL)P5-(5'-THYMIDYL)PENTAPHOSPHATE'>T5A</scene>, <scene name='pdbligand=THM:THYMIDINE'>THM</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span> |
| - | |GENE= TK OR UL23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= TK OR UL23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10298 Human herpesvirus 1]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1p75|1P75]], [[1p72|1P72]], [[1p6x|1P6X]], [[1p73|1P73]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p7c OCA], [http://www.ebi.ac.uk/pdbsum/1p7c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p7c RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1P7C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1P7C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_1 Human herpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7C OCA]. |
==Reference== | ==Reference== | ||
Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases., Gardberg A, Shuvalova L, Monnerjahn C, Konrad M, Lavie A, Structure. 2003 Oct;11(10):1265-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14527394 14527394] | Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases., Gardberg A, Shuvalova L, Monnerjahn C, Konrad M, Lavie A, Structure. 2003 Oct;11(10):1265-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14527394 14527394] | ||
| - | [[Category: Human herpesvirus | + | [[Category: Human herpesvirus 1]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thymidine kinase]] | [[Category: Thymidine kinase]] | ||
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[[Category: Monnerjahn, C.]] | [[Category: Monnerjahn, C.]] | ||
[[Category: Shuvalova, L.]] | [[Category: Shuvalova, L.]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: T5A]] | ||
| - | [[Category: THM]] | ||
[[Category: bisubstrate inhibitor]] | [[Category: bisubstrate inhibitor]] | ||
[[Category: lid]] | [[Category: lid]] | ||
[[Category: p-loop]] | [[Category: p-loop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:55:56 2008'' |
Revision as of 19:56, 30 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | TK OR UL23 (Human herpesvirus 1) | ||||||
| Activity: | Thymidine kinase, with EC number 2.7.1.21 | ||||||
| Related: | 1P75, 1P72, 1P6X, 1P73
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of HSV1-TK complexed with TP5A
Overview
Crystal structures of equine herpesvirus type-4 thymidine kinase (EHV4-TK) in complex with (i). thymidine and ADP, (ii). thymidine and SO(4) and the bisubstrate analogs, (iii). TP(4)A, and (iv). TP(5)A have been solved. Additionally, the structure of herpes simplex virus type-1 thymidine kinase (HSV1-TK) in complex with TP(5)A has been determined. These are the first structures of nucleoside kinases revealing conformational transitions upon binding of bisubstrate analogs. The structural basis for the dual thymidine and thymidylate kinase activity of these TKs is elucidated. While the active sites of HSV1-TK and EHV4-TK resemble one another, notable differences are observed in the Lid regions and in the way the enzymes bind the base of the phosphoryl-acceptor. The latter difference could partly explain the higher activity of EHV4-TK toward the prodrug ganciclovir.
About this Structure
1P7C is a Single protein structure of sequence from Human herpesvirus 1. Full crystallographic information is available from OCA.
Reference
Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases., Gardberg A, Shuvalova L, Monnerjahn C, Konrad M, Lavie A, Structure. 2003 Oct;11(10):1265-77. PMID:14527394
Page seeded by OCA on Sun Mar 30 22:55:56 2008
