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2lot
From Proteopedia
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==AR55 solubilised in SDS micelles== | ==AR55 solubilised in SDS micelles== | ||
<StructureSection load='2lot' size='340' side='right' caption='[[2lot]], [[NMR_Ensembles_of_Models | 40 NMR models]]' scene=''> | <StructureSection load='2lot' size='340' side='right' caption='[[2lot]], [[NMR_Ensembles_of_Models | 40 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2lot]] is a 1 chain structure | + | <table><tr><td colspan='2'>[[2lot]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LOT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LOT FirstGlance]. <br> |
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lou|2lou]], [[2lov|2lov]], [[2low|2low]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lou|2lou]], [[2lov|2lov]], [[2low|2low]]</td></tr> | ||
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lot OCA], [http://pdbe.org/2lot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lot RCSB], [http://www.ebi.ac.uk/pdbsum/2lot PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lot ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lot OCA], [http://pdbe.org/2lot PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lot RCSB], [http://www.ebi.ac.uk/pdbsum/2lot PDBsum]</span></td></tr> | + | |
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/APJ_HUMAN APJ_HUMAN]] Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. | [[http://www.uniprot.org/uniprot/APJ_HUMAN APJ_HUMAN]] Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Detergent micelles are frequently employed as membrane mimetics for solution-state membrane protein nuclear magnetic resonance spectroscopy. Here we compare topology, structure, ps-ns time-scale dynamics, and hydrodynamics of a model protein with one transmembrane (TM) segment (residues 1-55 of the apelin receptor, APJ, a G-protein-coupled receptor) in three distinct, commonly used micellar environments. In each environment, two solvent-protected helical segments connected by a solvent-exposed kink were observed. The break in helical character at the kink was maintained in a helix-stabilizing fluorinated alcohol environment, implying that this structural feature is inherent. Molecular dynamics simulations also substantiate favorable self-assembly of compact protein-micelle complexes with a more dynamic, solvent-exposed kink. Despite the observed similarity in TM segment behavior, micelle-dependent differences were clear in the structure, dynamics, and compactness of the 30-residue, extramembrane N-terminal tail of the protein. This would affect intermolecular interactions and, correspondingly, the functional state of the membrane protein. | ||
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| + | Preserved Transmembrane Segment Topology, Structure, and Dynamics in Disparate Micellar Environments.,Langelaan DN, Pandey A, Sarker M, Rainey JK J Phys Chem Lett. 2017 May 12:2381-2386. doi: 10.1021/acs.jpclett.7b00867. PMID:28492329<ref>PMID:28492329</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2lot" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Human]] | ||
[[Category: Langelaan, D N]] | [[Category: Langelaan, D N]] | ||
[[Category: Rainey, J K]] | [[Category: Rainey, J K]] | ||
[[Category: Membrane protein]] | [[Category: Membrane protein]] | ||
Revision as of 07:21, 24 May 2017
AR55 solubilised in SDS micelles
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