1ufj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 4: Line 4:
|PDB= 1ufj |SIZE=350|CAPTION= <scene name='initialview01'>1ufj</scene>, resolution 1.60&Aring;
|PDB= 1ufj |SIZE=350|CAPTION= <scene name='initialview01'>1ufj</scene>, resolution 1.60&Aring;
|SITE=
|SITE=
-
|LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=CZM:&#39;N,N&#39;-BIS-(2-HYDROXY-3-METHYL-BENZYLIDENE)-BENZENE-1,2-DIAMINE&#39;'>CZM</scene>
+
|LIGAND= <scene name='pdbligand=CZM:&#39;N,N&#39;-BIS-(2-HYDROXY-3-METHYL-BENZYLIDENE)-BENZENE-1,2-DIAMINE&#39;'>CZM</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
 +
|DOMAIN=
 +
|RELATEDENTRY=
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ufj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ufj OCA], [http://www.ebi.ac.uk/pdbsum/1ufj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ufj RCSB]</span>
}}
}}
Line 29: Line 32:
[[Category: Watanabe, Y.]]
[[Category: Watanabe, Y.]]
[[Category: Yamane, T.]]
[[Category: Yamane, T.]]
-
[[Category: CZM]]
 
-
[[Category: FE]]
 
-
[[Category: PO4]]
 
[[Category: iron]]
[[Category: iron]]
[[Category: metalloprotein]]
[[Category: metalloprotein]]
Line 38: Line 38:
[[Category: schiff base]]
[[Category: schiff base]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 13:51:52 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:09:35 2008''

Revision as of 21:09, 30 March 2008

Image:1ufj.gif


PDB ID 1ufj

Drag the structure with the mouse to rotate
, resolution 1.60Å
Ligands: , ,
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of an Artificial Metalloprotein:Fe(III)(3,3'-Me2-salophen)/apo-A71G Myoglobin


Overview

Apo-myoglobin (apo-Mb) and apo-A71GMb were successfully reconstituted with FeIII(salophen) (1) (salophen = N,N'-bis(salicylidene)-1,2-phenilenediamine), Fe(III)(3,3'-Me2-salophen) (2), and FeIII(5,5'-t-Bu2-salophen) (3). The crystal structure of 2.apo-A71GMb shows the tight binding of the complex in the Mb cavity, while in wild-type apo-Mb it is highly disordered due to the steric repulsion with Ala71. Furthermore, the structure of 2.apo-A71GMb suggests a possible accommodation of a small substrate in the cavity. In fact, the cyanide association rate constant of 2.apo-A71GMb is 216-fold larger compared to that of 2.apo-Mb. These results provide us principles for the noncovalent fixation of synthetic metal cofactors at the desired positions in protein matrixes.

About this Structure

1UFJ is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

Reference

Crystal structures of artificial metalloproteins: tight binding of FeIII(Schiff-Base) by mutation of Ala71 to Gly in apo-myoglobin., Ueno T, Ohashi M, Kono M, Kondo K, Suzuki A, Yamane T, Watanabe Y, Inorg Chem. 2004 May 3;43(9):2852-8. PMID:15106972

Page seeded by OCA on Mon Mar 31 00:09:35 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ufj"
Personal tools