1uon
From Proteopedia
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|PDB= 1uon |SIZE=350|CAPTION= <scene name='initialview01'>1uon</scene> | |PDB= 1uon |SIZE=350|CAPTION= <scene name='initialview01'>1uon</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=CH1:3'-DEOXY-CYTIDINE-5'-TRIPHOSPHATE'>CH1</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uon OCA], [http://www.ebi.ac.uk/pdbsum/1uon PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uon RCSB]</span> | ||
}} | }} | ||
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[[Category: Walker, S B.]] | [[Category: Walker, S B.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
| - | [[Category: CH1]] | ||
| - | [[Category: MN]] | ||
[[Category: core protein]] | [[Category: core protein]] | ||
[[Category: cryoem]] | [[Category: cryoem]] | ||
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[[Category: reovirus]] | [[Category: reovirus]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:13:10 2008'' |
Revision as of 21:13, 30 March 2008
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| Ligands: | , , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
REOVIRUS POLYMERASE LAMBDA-3 LOCALIZED BY ELECTRON CRYOMICROSCOPY OF VIRIONS AT 7.6-A RESOLUTION
Overview
Reovirus is an icosahedral, double-stranded (ds) RNA virus that uses viral polymerases packaged within the viral core to transcribe its ten distinct plus-strand RNAs. To localize these polymerases, the structure of the reovirion was refined to a resolution of 7.6 A by cryo-electron microscopy (cryo-EM) and three-dimensional (3D) image reconstruction. X-ray crystal models of reovirus proteins, including polymerase lambda 3, were then fitted into the density map. Each copy of lambda 3 was found anchored to the inner surface of the icosahedral core shell, making major contacts with three molecules of shell protein lambda 1 and overlapping, but not centering on, a five-fold axis. The overlap explains why only one copy of lambda 3 is bound per vertex. lambda 3 is furthermore oriented with its transcript exit channel facing a small channel through the lambda 1 shell, suggesting how the nascent RNA is passed into the large external cavity of the pentameric capping enzyme complex formed by protein lambda 2.
About this Structure
1UON is a Protein complex structure of sequences from Reovirus sp.. Full crystallographic information is available from OCA.
Reference
Reovirus polymerase lambda 3 localized by cryo-electron microscopy of virions at a resolution of 7.6 A., Zhang X, Walker SB, Chipman PR, Nibert ML, Baker TS, Nat Struct Biol. 2003 Dec;10(12):1011-8. Epub 2003 Nov 9. PMID:14608373
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