2lbv

From Proteopedia

(Difference between revisions)

Revision as of 19:18, 1 August 2018

Siderocalin Q83 reveals a dual ligand binding mode

Structural highlights

2lbv is a 1 chain structure with sequence from Cotja. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[EXFAB_COTJA] Siderocalin-like lipocalin tightly binding a variety of bacterial ferric siderophores, also binds long-chain unsaturated fatty acids such as linoleic acid, oleic acid, arachidonic acid and, with a lower affinity, long chain saturated fatty acids such as steraic acid. May act as an antibacterial factor, through dual ligand specificity, both as a siderophore-sequestrating molecule and a lysophosphatidic acid (LPA) sensor.^[1] ^[2]

Publication Abstract from PubMed

Siderocalins are particular lipocalins that participate in the innate immune response by interfering with bacterial siderophore-mediated iron uptake. Additionally, siderocalins are involved in several physiological and pathological processes such as inflammation, iron delivery, tissue differentiation, and cancer progression. Here we show that siderocalin Q83 displays an unexpected dual ligand binding mode as it can bind enterobactin and unsaturated fatty acids simultaneously. The solution structure of the siderocalin Q83 in complex with arachidonic acid and enterobactin reveals molecular details of this novel dual binding mode and the determinants of fatty acid binding specificity. Our results suggest that Q83 is a metabolic hub linking iron and fatty acid pathways. This unexpected coupling might contribute to the pleiotropic functions of siderocalins.

Lipocalin Q83 reveals a dual ligand binding mode with potential implications for the functions of siderocalins.,Coudevylle N, Hoetzinger M, Geist L, Kontaxis G, Hartl M, Bister K, Konrat R Biochemistry. 2011 Nov 1;50(43):9192-9. Epub 2011 Oct 7. PMID:21951132^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Coudevylle N, Geist L, Hotzinger M, Hartl M, Kontaxis G, Bister K, Konrat R. The v-myc-induced Q83 lipocalin is a siderocalin. J Biol Chem. 2010 Dec 31;285(53):41646-52. Epub 2010 Sep 8. PMID:20826777 doi:10.1074/jbc.M110.123331
↑ Coudevylle N, Hoetzinger M, Geist L, Kontaxis G, Hartl M, Bister K, Konrat R. Lipocalin Q83 reveals a dual ligand binding mode with potential implications for the functions of siderocalins. Biochemistry. 2011 Nov 1;50(43):9192-9. Epub 2011 Oct 7. PMID:21951132 doi:http://dx.doi.org/10.1021/bi201115q
↑ Coudevylle N, Hoetzinger M, Geist L, Kontaxis G, Hartl M, Bister K, Konrat R. Lipocalin Q83 reveals a dual ligand binding mode with potential implications for the functions of siderocalins. Biochemistry. 2011 Nov 1;50(43):9192-9. Epub 2011 Oct 7. PMID:21951132 doi:http://dx.doi.org/10.1021/bi201115q

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lbv"

@@ Line 1: / Line 1: @@
 ==Siderocalin Q83 reveals a dual ligand binding mode==
 <StructureSection load='2lbv' size='340' side='right' caption='[[2lbv]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2lbv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cotja Cotja]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LBV FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACD:ARACHIDONIC+ACID'>ACD</scene>, <scene name='pdbligand=EB4:N,N,N-[(3S,7S,11S)-2,6,10-TRIOXO-1,5,9-TRIOXACYCLODODECANE-3,7,11-TRIYL]TRIS(2,3-DIHYDROXYBENZAMIDE)'>EB4</scene>, <scene name='pdbligand=GA:GALLIUM+(III)+ION'>GA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lbv OCA], [http://pdbe.org/2lbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lbv RCSB], [http://www.ebi.ac.uk/pdbsum/2lbv PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lbv OCA], [http://pdbe.org/2lbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lbv RCSB], [http://www.ebi.ac.uk/pdbsum/2lbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lbv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 19: / Line 20: @@
 ==See Also==
-*[[Fatty acid-binding protein|Fatty acid-binding protein]]
+*[[Siderocalin|Siderocalin]]
 == References ==
 <references/>

2lbv

From Proteopedia

Revision as of 19:18, 1 August 2018

Siderocalin Q83 reveals a dual ligand binding mode

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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