2lnz

From Proteopedia

(Difference between revisions)

Revision as of 19:43, 1 August 2018

Solution structure of the Get5 carboxyl domain from S. cerevisiae

Structural highlights

2lnz is a 2 chain structure with sequence from Baker's yeast. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3vej, 2lo0
Gene:	GET5, MDY2, TMA24, YOL111C (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MDY2_YEAST] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.^[1] ^[2]

Publication Abstract from PubMed

Tail-anchored trans-membrane proteins are targeted to membranes post-translationally. The proteins Get4 and Get5 form an obligate complex that catalyzes the transfer of tail-anchored proteins destined to the endoplasmic reticulum from Sgt2 to the cytosolic targeting factor Get3. Get5 forms a homodimer mediated by its carboxyl domain. We show here that a conserved motif exists within the carboxyl domain. A high resolution crystal structure and solution NMR structures of this motif reveal a novel and stable helical dimerization domain. We additionally determined a solution NMR structure of a divergent fungal homolog, and comparison of these structures allows annotation of specific stabilizing interactions. Using solution x-ray scattering and the structures of all folded domains, we present a model of the full-length Get4/Get5 complex.

Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.,Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Iwanejko L, Smith KN, Loeillet S, Nicolas A, Fabre F. Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast. 1999 Oct;15(14):1529-39. PMID:10514570 doi:<1529::AID-YEA457>3.0.CO;2-Y 10.1002/(SICI)1097-0061(199910)15:14<1529::AID-YEA457>3.0.CO;2-Y
↑ Hu Z, Potthoff B, Hollenberg CP, Ramezani-Rad M. Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae. J Cell Sci. 2006 Jan 15;119(Pt 2):326-38. Epub 2006 Jan 3. PMID:16390866 doi:jcs.02754
↑ Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr. Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex. J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836 doi:10.1074/jbc.M111.333252

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lnz"

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 ==Solution structure of the Get5 carboxyl domain from S. cerevisiae==
 <StructureSection load='2lnz' size='340' side='right' caption='[[2lnz]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vej|3vej]], [[2lo0|2lo0]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GET5, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnz OCA], [http://pdbe.org/2lnz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lnz RCSB], [http://www.ebi.ac.uk/pdbsum/2lnz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnz OCA], [http://pdbe.org/2lnz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lnz RCSB], [http://www.ebi.ac.uk/pdbsum/2lnz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnz ProSAT]</span></td></tr>
 </table>
 == Function ==

2lnz

From Proteopedia

Revision as of 19:43, 1 August 2018

Solution structure of the Get5 carboxyl domain from S. cerevisiae

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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