2eu8
From Proteopedia
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|PDB= 2eu8 |SIZE=350|CAPTION= <scene name='initialview01'>2eu8</scene>, resolution 1.800Å | |PDB= 2eu8 |SIZE=350|CAPTION= <scene name='initialview01'>2eu8</scene>, resolution 1.800Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=AP5:BIS(ADENOSINE)-5'-PENTAPHOSPHATE'>AP5</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] </span> |
|GENE= adk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |GENE= adk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zin|1zin]], [[1p3j|1p3j]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eu8 OCA], [http://www.ebi.ac.uk/pdbsum/2eu8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2eu8 RCSB]</span> | ||
}} | }} | ||
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[[Category: Chen, S.]] | [[Category: Chen, S.]] | ||
[[Category: Shamoo, Y.]] | [[Category: Shamoo, Y.]] | ||
| - | [[Category: AP5]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: ZN]] | ||
[[Category: adenylate kinase]] | [[Category: adenylate kinase]] | ||
[[Category: in vivo evolution]] | [[Category: in vivo evolution]] | ||
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[[Category: thermostability]] | [[Category: thermostability]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:53:51 2008'' |
Revision as of 23:53, 30 March 2008
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| , resolution 1.800Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | adk (Bacillus subtilis) | ||||||
| Activity: | Adenylate kinase, with EC number 2.7.4.3 | ||||||
| Related: | 1zin, 1p3j
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a thermostable mutant of Bacillus subtilis Adenylate Kinase (Q199R)
Overview
In nature, evolution occurs through the continuous adaptation of a population to its environment. At the molecular level, adaptive changes in protein sequence and expression impact organismal fitness and, consequently, dictate population dynamics. Here, we have used a "weak link" method to favor variations in one gene, allowing adaptation to thermostability to be studied in molecular detail as bacteria were grown continuously for approximately 1500 generations. Surprisingly, only six mutant alleles, representing less than 1% of the possible missense mutations, were observed, suggesting a highly constrained molecular landscape during protein evolution. The changes in organismal fitness were linked directly to incremental increases in enzyme stability and activity maxima and corresponded to the narrow temperature ranges where each mutant enjoyed success within the overall population. Thus, continuous evolution of a single gene permits a quantitative approach that extends from the phenotypes of the microbial populations to their underlying biophysical basis.
About this Structure
2EU8 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
In vivo molecular evolution reveals biophysical origins of organismal fitness., Counago R, Chen S, Shamoo Y, Mol Cell. 2006 May 19;22(4):441-9. PMID:16713575
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