2gsz
From Proteopedia
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|PDB= 2gsz |SIZE=350|CAPTION= <scene name='initialview01'>2gsz</scene>, resolution 4.20Å | |PDB= 2gsz |SIZE=350|CAPTION= <scene name='initialview01'>2gsz</scene>, resolution 4.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= pilT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |GENE= pilT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2eyu|2EYU]], [[2ewv|2EWV]], [[2eww|2EWW]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsz OCA], [http://www.ebi.ac.uk/pdbsum/2gsz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gsz RCSB]</span> | ||
}} | }} | ||
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[[Category: Forest, K T.]] | [[Category: Forest, K T.]] | ||
[[Category: Satyshur, K A.]] | [[Category: Satyshur, K A.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: SO4]] | ||
[[Category: atpase]] | [[Category: atpase]] | ||
[[Category: domain motion]] | [[Category: domain motion]] | ||
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[[Category: reca]] | [[Category: reca]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:21:14 2008'' |
Revision as of 00:21, 31 March 2008
| |||||||
| , resolution 4.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | pilT (Aquifex aeolicus) | ||||||
| Related: | 2EYU, 2EWV, 2EWW
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of A. aeolicus PilT with 6 monomers per asymmetric unit
Overview
PilT is a hexameric ATPase required for bacterial type IV pilus retraction and surface motility. Crystal structures of ADP- and ATP-bound Aquifex aeolicus PilT at 2.8 and 3.2 A resolution show N-terminal PAS-like and C-terminal RecA-like ATPase domains followed by a set of short C-terminal helices. The hexamer is formed by extensive polar subunit interactions between the ATPase core of one monomer and the N-terminal domain of the next. An additional structure captures a nonsymmetric PilT hexamer in which approach of invariant arginines from two subunits to the bound nucleotide forms an enzymatically competent active site. A panel of pilT mutations highlights the importance of the arginines, the PAS-like domain, the polar subunit interface, and the C-terminal helices for retraction. We present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this hexameric motor. Our conclusions apply to the entire type II/IV secretion ATPase family.
About this Structure
2GSZ is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility., Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT, Structure. 2007 Mar;15(3):363-76. PMID:17355871
Page seeded by OCA on Mon Mar 31 03:21:14 2008
Categories: Aquifex aeolicus | Single protein | Forest, K T. | Satyshur, K A. | Atpase | Domain motion | P-loop | Pa | Reca
