2h1f
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= rfaC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=217992 Escherichia coli O6]) | |GENE= rfaC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=217992 Escherichia coli O6]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2gt1|2GT1]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1f OCA], [http://www.ebi.ac.uk/pdbsum/2h1f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h1f RCSB]</span> | ||
}} | }} | ||
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[[Category: Vincent, S.]] | [[Category: Vincent, S.]] | ||
[[Category: Vongsouthi, V.]] | [[Category: Vongsouthi, V.]] | ||
| - | [[Category: ADP]] | ||
[[Category: gt-b fold absence of c-terminal alpha-helix]] | [[Category: gt-b fold absence of c-terminal alpha-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:24:10 2008'' |
Revision as of 00:24, 31 March 2008
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| , resolution 2.400Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | rfaC (Escherichia coli O6) | ||||||
| Related: | 2GT1
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
E. coli heptosyltransferase WaaC with ADP
Overview
Lipopolysaccharides constitute the outer leaflet of the outer membrane of Gram-negative bacteria and are therefore essential for cell growth and viability. The heptosyltransferase WaaC is a glycosyltransferase (GT) involved in the synthesis of the inner core region of LPS. It catalyzes the addition of the first L-glycero-D-manno-heptose (heptose) molecule to one 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residue of the Kdo2-lipid A molecule. Heptose is an essential component of the LPS core domain; its absence results in a truncated lipopolysaccharide associated with the deep-rough phenotype causing a greater susceptibility to antibiotic and an attenuated virulence for pathogenic Gram-negative bacteria. Thus, WaaC represents a promising target in antibacterial drug design. Here, we report the structure of WaaC from the Escherichia coli pathogenic strain RS218 alone at 1.9 A resolution, and in complex with either ADP or the non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at 2.4 A resolution. WaaC adopts the GT-B fold in two domains, characteristic of one glycosyltransferase structural superfamily. The comparison of the three different structures shows that WaaC does not undergo a domain rotation, characteristic of the GT-B family, upon substrate binding, but allows the substrate analog and the reaction product to adopt remarkably distinct conformations inside the active site. In addition, both binary complexes offer a close view of the donor subsite and, together with results from site-directed mutagenesis studies, provide evidence for a model of the catalytic mechanism.
About this Structure
2H1F is a Single protein structure of sequence from Escherichia coli o6. Full crystallographic information is available from OCA.
Reference
Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose., Grizot S, Salem M, Vongsouthi V, Durand L, Moreau F, Dohi H, Vincent S, Escaich S, Ducruix A, J Mol Biol. 2006 Oct 20;363(2):383-94. Epub 2006 Jul 29. PMID:16963083
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