2nmv
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2nmv |SIZE=350|CAPTION= <scene name='initialview01'>2nmv</scene>, resolution 2.95Å | |PDB= 2nmv |SIZE=350|CAPTION= <scene name='initialview01'>2nmv</scene>, resolution 2.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=FLU:2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-BENZOIC+ACID'>FLU</scene>, <scene name='pdbligand=NML:N-METHYLACETAMIDE'>NML</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= uvrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |GENE= uvrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2d7d|2D7D]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nmv OCA], [http://www.ebi.ac.uk/pdbsum/2nmv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nmv RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Geddes, S.]] | [[Category: Geddes, S.]] | ||
[[Category: Waters, T R.]] | [[Category: Waters, T R.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: FLU]] | ||
| - | [[Category: NML]] | ||
[[Category: hairpin]] | [[Category: hairpin]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
[[Category: t-fluorescein]] | [[Category: t-fluorescein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:05:18 2008'' |
Revision as of 01:05, 31 March 2008
| |||||||
| , resolution 2.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | uvrB (Bacillus subtilis) | ||||||
| Related: | 2D7D
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA
Overview
UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide.
About this Structure
2NMV is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA., Waters TR, Eryilmaz J, Geddes S, Barrett TE, FEBS Lett. 2006 Nov 27;580(27):6423-7. Epub 2006 Nov 3. PMID:17097086
Page seeded by OCA on Mon Mar 31 04:05:18 2008
