3bd9
From Proteopedia
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|SITE= <scene name='pdbsite=AC1:A3p+Binding+Site+For+Residue+A+347'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:A3p+Binding+Site+For+Residue+A+347'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3'-5'-DIPHOSPHATE'>A3P</scene> | |LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3'-5'-DIPHOSPHATE'>A3P</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/[Heparan_sulfate]-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.23 2.8.2.23] </span> |
|GENE= HS3ST5, 3OST5, HS3OST5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= HS3ST5, 3OST5, HS3OST5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bd9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bd9 OCA], [http://www.ebi.ac.uk/pdbsum/3bd9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bd9 RCSB]</span> | ||
}} | }} | ||
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[[Category: Song, D.]] | [[Category: Song, D.]] | ||
[[Category: Xu, D.]] | [[Category: Xu, D.]] | ||
| - | [[Category: A3P]] | ||
[[Category: 3-o-sulfotransferase]] | [[Category: 3-o-sulfotransferase]] | ||
[[Category: glycoprotein]] | [[Category: glycoprotein]] | ||
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[[Category: transmembrane]] | [[Category: transmembrane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:25:19 2008'' |
Revision as of 02:25, 31 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Gene: | HS3ST5, 3OST5, HS3OST5 (Homo sapiens) | ||||||
| Activity: | [Heparan_sulfate-glucosamine_3-sulfotransferase_1 [Heparan sulfate]-glucosamine 3-sulfotransferase 1], with EC number 2.8.2.23 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
human 3-O-sulfotransferase isoform 5 with bound PAP
Overview
The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions.
About this Structure
3BD9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Engineering sulfotransferases to modify heparan sulfate., Xu D, Moon AF, Song D, Pedersen LC, Liu J, Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:18223645[[Category: [Heparan sulfate]-glucosamine 3-sulfotransferase 1]]
Page seeded by OCA on Mon Mar 31 05:25:19 2008
