This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3gsp
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 3gsp |SIZE=350|CAPTION= <scene name='initialview01'>3gsp</scene>, resolution 1.9Å | |PDB= 3gsp |SIZE=350|CAPTION= <scene name='initialview01'>3gsp</scene>, resolution 1.9Å | ||
|SITE= <scene name='pdbsite=BI1:Site'>BI1</scene>, <scene name='pdbsite=CAL:Site'>CAL</scene> and <scene name='pdbsite=CAT:Active+Site'>CAT</scene> | |SITE= <scene name='pdbsite=BI1:Site'>BI1</scene>, <scene name='pdbsite=CAL:Site'>CAL</scene> and <scene name='pdbsite=CAT:Active+Site'>CAT</scene> | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=3GP:GUANOSINE-3'-MONOPHOSPHATE'>3GP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SGP:GUANOSINE-2',3'-CYCLOPHOSPHOROTHIOATE'>SGP</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gsp OCA], [http://www.ebi.ac.uk/pdbsum/3gsp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3gsp RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Wyns, L.]] | [[Category: Wyns, L.]] | ||
[[Category: Zegers, I.]] | [[Category: Zegers, I.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: SGP]] | ||
[[Category: endoribonuclease]] | [[Category: endoribonuclease]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:33:44 2008'' |
Revision as of 02:33, 31 March 2008
| |||||||
| , resolution 1.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , and | ||||||
| Ligands: | , , | ||||||
| Activity: | Ribonuclease T(1), with EC number 3.1.27.3 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RIBONUCLEASE T1 COMPLEXED WITH 2',3'-CGPS + 3'-GMP, 4 DAYS
Overview
Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.
About this Structure
3GSP is a Single protein structure of sequence from Aspergillus oryzae. Full crystallographic information is available from OCA.
Reference
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography., Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L, Nat Struct Biol. 1998 Apr;5(4):280-3. PMID:9546218
Page seeded by OCA on Mon Mar 31 05:33:44 2008
