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2l46

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Revision as of 19:20, 1 August 2018

C-terminal zinc finger of the HIVNCp7 with platinated DNA

Structural highlights

2l46 is a 2 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2l44, 2l45
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q9YP46_9HIV1] Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity).[SAAS:SAAS012344_004_008806] Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).[SAAS:SAAS012344_004_011858]

Publication Abstract from PubMed

This paper describes for the first time the intimate molecular details of the association between a platinated oligonucleotide and a zinc finger peptide. Site-specific platination of the guanine in a single-stranded hexanucleotide gave {[Pt(dien)d(5'-TACGCC-3')], Pt(dien)(6-mer)} (II) characterized by mass spectrometry and (1)H nuclear magnetic resonance (NMR) spectroscopy. The work extends the study of platinum-nucleobase complex-zinc finger interactions using small molecules such as [Pt(dien)(9-EtGua)](2+) (I). The structure of the (34-52) C-terminal finger of HIV nucleocapsid protein HIVNCp7 (ZF1) was characterized by (1)H NMR spectroscopy and compared with that of the N-terminal single finger and the two-finger "intact" NCp7. Interaction of II with ZF1 results in significant changes in comparison to the "free" uncomplexed hexanucleotide; the major changes occurring for Trp37 resonances that are broadened and moved upfield, and other major shifts are for Gln45 (Hepsilon21, Hgamma3, Qbeta), Met46 (NH, Hgamma2), Lys47 (NH, Qgamma), and Glu50 (Hgamma2, Hgamma3). The Zn-Cys/His chemical shifts show only marginal deviations. The solution structures of ZF1 and the 6-mer-ZF1 and II-ZF1 adducts were calculated from the nuclear Overhauser effect spectroscopy-derived distance constraints. The DNA position in the II-ZF1 adduct is completely different than in the absence of platinum. Major differences are the appearance of new Met46-Cyt6 H5 and Trp37-Cyt5 H5 contacts but severe weakening of the Trp37-Gua4 contact, attributed to the steric effects caused by Gua4 platination, accompanied by a change in the position of the aromatic ring. The results demonstrate the feasibility of targeting specific ZF motifs with DNA-tethered coordination compounds, such as Pt compounds and Co macrocycles, with implications for drug targetting and indeed the intimate mechanisms of DNA repair of platinated DNA.

Platinated DNA affects zinc finger conformation. Interaction of a platinated single-stranded oligonucleotide and the C-terminal zinc finger of nucleocapsid protein HIVNCp7.,Quintal S, Viegas A, Erhardt S, Cabrita EJ, Farrell NP Biochemistry. 2012 Feb 28;51(8):1752-61. Epub 2012 Feb 14. PMID:22303928^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Quintal S, Viegas A, Erhardt S, Cabrita EJ, Farrell NP. Platinated DNA affects zinc finger conformation. Interaction of a platinated single-stranded oligonucleotide and the C-terminal zinc finger of nucleocapsid protein HIVNCp7. Biochemistry. 2012 Feb 28;51(8):1752-61. Epub 2012 Feb 14. PMID:22303928 doi:10.1021/bi201834g

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2l46"

@@ Line 1: / Line 1: @@
 ==C-terminal zinc finger of the HIVNCp7 with platinated DNA==
 <StructureSection load='2l46' size='340' side='right' caption='[[2l46]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2l44|2l44]], [[2l45|2l45]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l46 OCA], [http://pdbe.org/2l46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l46 RCSB], [http://www.ebi.ac.uk/pdbsum/2l46 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l46 OCA], [http://pdbe.org/2l46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l46 RCSB], [http://www.ebi.ac.uk/pdbsum/2l46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l46 ProSAT]</span></td></tr>
 </table>
 == Function ==

2l46

From Proteopedia

Revision as of 19:20, 1 August 2018

C-terminal zinc finger of the HIVNCp7 with platinated DNA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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