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2hf4

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Revision as of 06:34, 27 June 2018

Crystal structure of Monomeric Actin in its ATP-bound state

Structural highlights

2hf4 is a 1 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACT1_DROME] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.

Publication Abstract from PubMed

Actin filament growth and disassembly, as well as affinity for actin-binding proteins, is mediated by the nucleotide-bound state of the component actin monomers. The structural differences between ATP-actin and ADP-actin, however, remain controversial. We expressed a cytoplasmic actin in Sf9 cells, which was rendered non-polymerizable by virtue of two point mutations in subdomain 4 (A204E/P243K). This homogeneous monomer, called AP-actin, was crystallized in the absence of toxins, binding proteins, or chemical modification, with ATP or ADP at the active site. The two surface mutations do not perturb the structure. Significant differences between the two states are confined to the active site region and sensor loop. The active site cleft remains closed in both states. Minor structural shifts propagate from the active site toward subdomain 2, but dissipate before reaching the DNase binding loop (D-loop), which remains disordered in both the ADP and ATP states. This result contrasts with previous structures of actin made monomeric by modification with tetramethylrhodamine, which show formation of an alpha-helix at the distal end of the D-loop in the ADP-bound but not the ATP-bound form (Otterbein, L. R., Graceffa, P., and Dominguez, R. (2001) Science 293, 708-711). Our reanalysis of the TMR-modified actin structures suggests that the nucleotide-dependent formation of the D-loop helix may result from signal propagation through crystal packing interactions. Whereas the observed nucleotide-dependent changes in the structure present significantly different surfaces on the exterior of the actin monomer, current models of the actin filament lack any actin-actin interactions that involve the region of these key structural changes.

Crystal structures of expressed non-polymerizable monomeric actin in the ADP and ATP states.,Rould MA, Wan Q, Joel PB, Lowey S, Trybus KM J Biol Chem. 2006 Oct 20;281(42):31909-19. Epub 2006 Aug 18. PMID:16920713^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rould MA, Wan Q, Joel PB, Lowey S, Trybus KM. Crystal structures of expressed non-polymerizable monomeric actin in the ADP and ATP states. J Biol Chem. 2006 Oct 20;281(42):31909-19. Epub 2006 Aug 18. PMID:16920713 doi:M601973200

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hf4"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Monomeric Actin in its ATP-bound state==
 <StructureSection load='2hf4' size='340' side='right' caption='[[2hf4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2hf4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2HF4 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hf4 OCA], [http://pdbe.org/2hf4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hf4 RCSB], [http://www.ebi.ac.uk/pdbsum/2hf4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hf4 OCA], [http://pdbe.org/2hf4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hf4 RCSB], [http://www.ebi.ac.uk/pdbsum/2hf4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hf4 ProSAT]</span></td></tr>
 </table>
 == Function ==

2hf4

From Proteopedia

Revision as of 06:34, 27 June 2018

Crystal structure of Monomeric Actin in its ATP-bound state

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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