8psh
From Proteopedia
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|PDB= 8psh |SIZE=350|CAPTION= <scene name='initialview01'>8psh</scene> | |PDB= 8psh |SIZE=350|CAPTION= <scene name='initialview01'>8psh</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=AS:2-DEOXY-ADENOSINE+-5'-THIO-MONOPHOSPHATE'>AS</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=GS:GUANOSINE-5'-THIO-MONOPHOSPHATE'>GS</scene>, <scene name='pdbligand=PST:THYMIDINE-5'-THIOPHOSPHATE'>PST</scene>, <scene name='pdbligand=SC:2-DEOXY-CYTIDINE-5'-THIOPHOSPHORATE'>SC</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[8drh|8DRH]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8psh OCA], [http://www.ebi.ac.uk/pdbsum/8psh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=8psh RCSB]</span> | ||
}} | }} | ||
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[[Category: Kessler, H.]] | [[Category: Kessler, H.]] | ||
[[Category: Kurz, G.]] | [[Category: Kurz, G.]] | ||
| - | [[Category: G]] | ||
[[Category: antisense]] | [[Category: antisense]] | ||
[[Category: dna/rna hybrid]] | [[Category: dna/rna hybrid]] | ||
| Line 34: | Line 36: | ||
[[Category: thionucleotide]] | [[Category: thionucleotide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:45:43 2008'' |
Revision as of 02:45, 31 March 2008
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| Ligands: | , , , , , , , | ||||||
| Related: | 8DRH
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE
Overview
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
About this Structure
8PSH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216
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