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1wvf

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Revision as of 16:14, 11 April 2018

p-Cresol Methylhydroxylase: Alteration of the Structure of the Flavoprotein Subunit upon its Binding to the Cytochrome Subunit

Structural highlights

1wvf is a 1 chain structure with sequence from "bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	1dii, 1diq, 1wve
Activity:	4-methylphenol dehydrogenase (hydroxylating), with EC number 1.17.99.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The structures of two forms of a recombinant flavoprotein have been determined at high resolution and compared. These proteins are (1) the flavocytochrome c p-cresol methylhydroxylase (rPCMH, 1.85 A resolution) and (2) the cytochrome-free flavoprotein subunit of rPCMH (PchF, 1.30 A resolution). A significant conformational difference is observed in a protein segment that is in contact with the re face of the isoalloxazine ring of FAD when the structure of PchF is compared to the subunit in the intact flavocytochrome. This structural change is important for optimum catalytic function of the flavoprotein, which has been shown to be dependent on the presence of the cytochrome subunit. This change results in different protein-flavin and apparently different protein-substrate interactions that have a "tuning effect" on the electronic and redox properties of bound p-cresol and the covalently bound FAD. The conformational change in the segment in the cofactor-binding site is induced by a small rearrangement in the flavoprotein-cytochrome interface region of the flavoprotein.

p-Cresol methylhydroxylase: alteration of the structure of the flavoprotein subunit upon its binding to the cytochrome subunit.,Cunane LM, Chen ZW, McIntire WS, Mathews FS Biochemistry. 2005 Mar 1;44(8):2963-73. PMID:15723539^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cunane LM, Chen ZW, McIntire WS, Mathews FS. p-Cresol methylhydroxylase: alteration of the structure of the flavoprotein subunit upon its binding to the cytochrome subunit. Biochemistry. 2005 Mar 1;44(8):2963-73. PMID:15723539 doi:10.1021/bi048020r

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wvf"

@@ Line 1: / Line 1: @@
 ==p-Cresol Methylhydroxylase: Alteration of the Structure of the Flavoprotein Subunit upon its Binding to the Cytochrome Subunit==
 <StructureSection load='1wvf' size='340' side='right' caption='[[1wvf]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dii|1dii]], [[1diq|1diq]], [[1wve|1wve]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/4-methylphenol_dehydrogenase_(hydroxylating) 4-methylphenol dehydrogenase (hydroxylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.99.1 1.17.99.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvf OCA], [http://pdbe.org/1wvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wvf RCSB], [http://www.ebi.ac.uk/pdbsum/1wvf PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wvf OCA], [http://pdbe.org/1wvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wvf RCSB], [http://www.ebi.ac.uk/pdbsum/1wvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wvf ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">

1wvf

From Proteopedia

Revision as of 16:14, 11 April 2018

p-Cresol Methylhydroxylase: Alteration of the Structure of the Flavoprotein Subunit upon its Binding to the Cytochrome Subunit

Structural highlights

Publication Abstract from PubMed

References

Contents

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