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2iqh

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Revision as of 07:51, 4 July 2018

Influenza A virus nucleoprotein NP at 3.2A resolution

Structural highlights

2iqh is a 3 chain structure with sequence from I33a0. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	NP (I33A0)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q1I2B5_9INFA] Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).[SAAS:SAAS00208116]

Publication Abstract from PubMed

Influenza A viruses pose a serious threat to world public health, particularly the currently circulating avian H5N1 viruses. The influenza viral nucleoprotein forms the protein scaffold of the helical genomic ribonucleoprotein complexes, and has a critical role in viral RNA replication. Here we report a 3.2 A crystal structure of this nucleoprotein, the overall shape of which resembles a crescent with a head and a body domain, with a protein fold different compared with that of the rhabdovirus nucleoprotein. Oligomerization of the influenza virus nucleoprotein is mediated by a flexible tail loop that is inserted inside a neighbouring molecule. This flexibility in the tail loop enables the nucleoprotein to form loose polymers as well as rigid helices, both of which are important for nucleoprotein functions. Single residue mutations in the tail loop result in the complete loss of nucleoprotein oligomerization. An RNA-binding groove, which is found between the head and body domains at the exterior of the nucleoprotein oligomer, is lined with highly conserved basic residues widely distributed in the primary sequence. The nucleoprotein structure shows that only one of two proposed nuclear localization signals are accessible, and suggests that the body domain of nucleoprotein contains the binding site for the viral polymerase. Our results identify the tail loop binding pocket as a potential target for antiviral development.

The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA.,Ye Q, Krug RM, Tao YJ Nature. 2006 Dec 21;444(7122):1078-82. Epub 2006 Dec 6. PMID:17151603^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ye Q, Krug RM, Tao YJ. The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA. Nature. 2006 Dec 21;444(7122):1078-82. Epub 2006 Dec 6. PMID:17151603 doi:nature05379

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iqh"

@@ Line 1: / Line 1: @@
 ==Influenza A virus nucleoprotein NP at 3.2A resolution==
 <StructureSection load='2iqh' size='340' side='right' caption='[[2iqh]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2iqh]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/I33a0 I33a0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IQH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IQH FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381518 I33A0])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iqh OCA], [http://pdbe.org/2iqh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iqh RCSB], [http://www.ebi.ac.uk/pdbsum/2iqh PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iqh OCA], [http://pdbe.org/2iqh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2iqh RCSB], [http://www.ebi.ac.uk/pdbsum/2iqh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2iqh ProSAT]</span></td></tr>
 </table>
 == Function ==

2iqh

From Proteopedia

Revision as of 07:51, 4 July 2018

Influenza A virus nucleoprotein NP at 3.2A resolution

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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