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1mzt

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Revision as of 10:10, 25 October 2017

NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes

Structural highlights

1mzt is a 1 chain structure with sequence from Enterobacteria phage fd. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1fdm
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAPSD_BPFD] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).

Publication Abstract from PubMed

A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor "shotgun," of (15)N-labeled amino acids throughout the protein sequence (and the resulting NMR spectra). The samples are obtained by protein expression in bacteria grown on media in which one type of amino acid is labeled and the others are not. Shotgun NMR short-circuits the laborious and time-consuming process of obtaining complete sequential assignments prior to the calculation of a protein structure from the NMR data by taking advantage of the orientational information inherent to the spectra of aligned proteins. As a result, it is possible to simultaneously assign resonances and measure orientational restraints for structure determination. A total of five two-dimensional (1)H/(15)N PISEMA (polarization inversion spin exchange at the magic angle) spectra, from one uniformly and four selectively (15)N-labeled samples, were sufficient to determine the structure of the membrane-bound form of the 50-residue major pVIII coat protein of fd filamentous bacteriophage. Pisa (polarity index slat angle) wheels are an essential element in the process, which starts with the simultaneous assignment of resonances and the assembly of isolated polypeptide segments, and culminates in the complete three-dimensional structure of the protein with atomic resolution. The principles are also applicable to weakly aligned proteins studied by solution NMR spectroscopy. [The structure we determined for the membrane-bound form of the Fd bacteriophage pVIII coat protein has been deposited in the Protein Data Bank as PDB file 1MZT.]

Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints.,Marassi FM, Opella SJ Protein Sci. 2003 Mar;12(3):403-11. PMID:12592011^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Marassi FM, Opella SJ. Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints. Protein Sci. 2003 Mar;12(3):403-11. PMID:12592011

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mzt"

@@ Line 1: / Line 1: @@
 ==NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes==
 <StructureSection load='1mzt' size='340' side='right' caption='[[1mzt]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpfd Bpfd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1mzt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_fd Enterobacteria phage fd]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1MZT FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fdm|1fdm]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://pdbe.org/1mzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mzt OCA], [http://pdbe.org/1mzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mzt RCSB], [http://www.ebi.ac.uk/pdbsum/1mzt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mzt ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Bpfd]]
+[[Category: Enterobacteria phage fd]]
 [[Category: Marassi, F M]]
 [[Category: Opella, S J]]

1mzt

From Proteopedia

Revision as of 10:10, 25 October 2017

NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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