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2kx7

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Revision as of 08:15, 25 July 2018

Solution structure of the E.coli RcsD-ABL domain (residues 688-795)

Structural highlights

2kx7 is a 1 chain structure with sequence from Ecoli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1sr2
Gene:	b2216, JW2204, yojN, yojP, yojQ (ECOLI)
Activity:	Histidine kinase, with EC number 2.7.13.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RCSD_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsD is a phosphotransfer intermediate between the sensor kinase RcsC and the response regulator RcsB. It acquires a phosphoryl group from RcsC and transfers it to RcsB. The system controls expression of genes involved in colanic acid capsule synthesis, biofilm formation and cell division.[HAMAP-Rule:MF_00980]^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The Rcs-signaling system is one of the most remarkable phosphorelay pathways in Enterobacteriaceae, comprising several membrane-bound and soluble proteins. Within the complex phosphotransfer pathway, the histidine phosphotransferase (HPt) domain of the RcsD membrane-bound component serves as a crucial factor in modulating the phosphorylation state of the transcription factor RcsB. We have identified a new domain, RcsD-ABL, located N terminally to RcsD-HPt that interacts with RcsB as well. We have determined its structure, characterized its interaction interface with RcsB, and built a structural model of the complex of the RcsD-ABL domain with RcsB. Our results indicate that the effector domain of RcsB, which normally binds to DNA, is recognized by RcsD-ABL, whereas the HPt domain interacts with the phosphoreceiver domain of RcsB.

Structural Insights into Rcs Phosphotransfer: The Newly Identified RcsD-ABL Domain Enhances Interaction with the Response Regulator RcsB.,Schmoe K, Rogov VV, Rogova NY, Lohr F, Guntert P, Bernhard F, Dotsch V Structure. 2011 Apr 13;19(4):577-87. PMID:21481780^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Carballes F, Bertrand C, Bouche JP, Cam K. Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two-component system rcsC-rcsB. Mol Microbiol. 1999 Nov;34(3):442-50. PMID:10564486
↑ Takeda S, Fujisawa Y, Matsubara M, Aiba H, Mizuno T. A novel feature of the multistep phosphorelay in Escherichia coli: a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour. Mol Microbiol. 2001 Apr;40(2):440-50. PMID:11309126
↑ Chen MH, Takeda S, Yamada H, Ishii Y, Yamashino T, Mizuno T. Characterization of the RcsC-->YojN-->RcsB phosphorelay signaling pathway involved in capsular synthesis in Escherichia coli. Biosci Biotechnol Biochem. 2001 Oct;65(10):2364-7. PMID:11758943
↑ Hagiwara D, Sugiura M, Oshima T, Mori H, Aiba H, Yamashino T, Mizuno T. Genome-wide analyses revealing a signaling network of the RcsC-YojN-RcsB phosphorelay system in Escherichia coli. J Bacteriol. 2003 Oct;185(19):5735-46. PMID:13129944
↑ Ferrieres L, Clarke DJ. The RcsC sensor kinase is required for normal biofilm formation in Escherichia coli K-12 and controls the expression of a regulon in response to growth on a solid surface. Mol Microbiol. 2003 Dec;50(5):1665-82. PMID:14651646
↑ Schmoe K, Rogov VV, Rogova NY, Lohr F, Guntert P, Bernhard F, Dotsch V. Structural Insights into Rcs Phosphotransfer: The Newly Identified RcsD-ABL Domain Enhances Interaction with the Response Regulator RcsB. Structure. 2011 Apr 13;19(4):577-87. PMID:21481780 doi:10.1016/j.str.2011.01.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kx7"

@@ Line 1: / Line 1: @@
 ==Solution structure of the E.coli RcsD-ABL domain (residues 688-795)==
 <StructureSection load='2kx7' size='340' side='right' caption='[[2kx7]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b2216, JW2204, yojN, yojP, yojQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kx7 OCA], [http://pdbe.org/2kx7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kx7 RCSB], [http://www.ebi.ac.uk/pdbsum/2kx7 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kx7 OCA], [http://pdbe.org/2kx7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kx7 RCSB], [http://www.ebi.ac.uk/pdbsum/2kx7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kx7 ProSAT]</span></td></tr>
 </table>
 == Function ==

2kx7

From Proteopedia

Revision as of 08:15, 25 July 2018

Solution structure of the E.coli RcsD-ABL domain (residues 688-795)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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