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2gia

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Revision as of 07:17, 20 June 2018

Crystal structures of trypanosoma bruciei MRP1/MRP2

Structural highlights

2gia is a 4 chain structure with sequence from Tryb2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2gid, 2gje
Gene:	mrp1 (TRYB2), mrp2 (TRYB2)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The mitochondrial RNA binding proteins MRP1 and MRP2 form a heteromeric complex that functions in kinetoplastid RNA editing. In this process, MRP1/MRP2 serves as a matchmaker by binding to guide RNAs and facilitating their hybridization with cognate preedited mRNAs. To understand the mechanism by which this complex performs RNA matchmaking, we determined structures of Trypanosoma brucei apoMRP1/MRP2 and an MRP1/MRP2-gRNA complex. The structures show that MRP1/MRP2 is a heterotetramer and, despite little sequence homology, each MRP subunit exhibits the same "Whirly" transcription-factor fold. The gRNA molecule binds to the highly basic beta sheet surface of the MRP complex via nonspecific, electrostatic contacts. Strikingly, while the gRNA stem/loop II base is anchored to the basic surface, stem/loop I (the anchor sequence) is unfolded and its bases exposed to solvent. Thus, MRP1/MRP2 acts as an RNA matchmaker by stabilizing the RNA molecule in an unfolded conformation suitable for RNA-RNA hybridization.

Crystal structures of T. brucei MRP1/MRP2 guide-RNA binding complex reveal RNA matchmaking mechanism.,Schumacher MA, Karamooz E, Zikova A, Trantirek L, Lukes J Cell. 2006 Aug 25;126(4):701-11. PMID:16923390^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schumacher MA, Karamooz E, Zikova A, Trantirek L, Lukes J. Crystal structures of T. brucei MRP1/MRP2 guide-RNA binding complex reveal RNA matchmaking mechanism. Cell. 2006 Aug 25;126(4):701-11. PMID:16923390 doi:S0092-8674(06)00964-0

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gia"

@@ Line 1: / Line 1: @@
 ==Crystal structures of trypanosoma bruciei MRP1/MRP2==
 <StructureSection load='2gia' size='340' side='right' caption='[[2gia]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gia]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_treu927 Trypanosoma brucei treu927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GIA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gia]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Tryb2 Tryb2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GIA FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gid|2gid]], [[2gje|2gje]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mrp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 Trypanosoma brucei TREU927]), mrp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 Trypanosoma brucei TREU927])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mrp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2]), mrp2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gia OCA], [http://pdbe.org/2gia PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gia RCSB], [http://www.ebi.ac.uk/pdbsum/2gia PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gia OCA], [http://pdbe.org/2gia PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gia RCSB], [http://www.ebi.ac.uk/pdbsum/2gia PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gia ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Trypanosoma brucei treu927]]
+[[Category: Tryb2]]
 [[Category: Karamooz, E]]
 [[Category: Lukes, J]]

2gia

From Proteopedia

Revision as of 07:17, 20 June 2018

Crystal structures of trypanosoma bruciei MRP1/MRP2

Structural highlights

Publication Abstract from PubMed

References

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